SHB Antibody (HL1455) - Azide and BSA Free

Western Blot: SHB Antibody (HL1455) - Azide and BSA Free [NBP3-25680] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SHB antibody [HL1455] (NBP3-25680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunohistochemistry-Paraffin: SHB Antibody (HL1455) - Azide and BSA Free [NBP3-25680] - SHB antibody [HL1455] detects SHB protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. SHB stained by SHB antibody [HL1455] (NBP3-25680) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu, Bv
• Applications: WB, IHC
• Clone: HL1455
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

SHB Antibody (HL1455) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit SHB Antibody (HL1455) - Azide and BSA Free (NBP3-25680) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SHB. The exact sequence is proprietary.
• Source: HEK293
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: SHB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for SHB Antibody (HL1455) - Azide and BSA Free

• bA3J10.2
• RP11-3J10.8
• SH2 domain-containing adapter protein B
• SHB (Src homology 2 domain containing) adaptor protein B
• SHB adaptor protein (a Src homology 2 protein)
• SHB
• Src homology 2 domain containing adaptor protein B

Background

SHB is an adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. It may play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. It may also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic-FGF and NGF-induced signaling cascades, and may also regulate IRS1 and IRS2 signaling in insulin-producing cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SHB