CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="SHB was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human SHB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7036) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF7036-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94662-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22624-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58223-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-30557-25ul | |||
![Western Blot: SHB Antibody (HL1455) - Azide and BSA Free [NBP3-25680] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SHB antibody [HL1455] (NBP3-25680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25680_rabbit-shb-mab-hl1455-azide-and-bsa-free-2120249355594.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: SHB Antibody (HL1455) - Azide and BSA Free [NBP3-25680] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SHB antibody [HL1455] (NBP3-25680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: SHB Antibody (HL1455) - Azide and BSA Free [NBP3-25680] - SHB antibody [HL1455] detects SHB protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. SHB stained by SHB antibody [HL1455] (NBP3-25680) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25680_rabbit-shb-mab-hl1455-azide-and-bsa-free-2120249425484.jpg)
![Western Blot: SHB Antibody (1M5C5) [NBP3-33468] - Western blot analysis of various lysates, using SHB Rabbit mAb at 1:600 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-33468_rabbit-shb-mab-1m5c5-91220241411283.jpg "Western Blot: SHB Antibody (1M5C5) [NBP3-33468] - Western blot analysis of various lysates, using SHB Rabbit mAb at 1:600 dilution.<br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.")
![Western Blot: SHB Antibody [NBP2-94662] - Analysis of extracts of various cell lines, using SHB at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.](http://images.novusbio.com/fullsize/SHB-Antibody-Western-Blot-NBP2-94662-img0001.jpg "Western Blot: SHB Antibody [NBP2-94662] - Analysis of extracts of various cell lines, using SHB at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 90s.")
![Western Blot: SHB Antibody (SR1974) [NBP3-22624] - Western blot analysis using NBP3-22624 in K562 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22624_rabbit-shb-mab-sr1974-572023153128.jpg "Western Blot: SHB Antibody (SR1974) [NBP3-22624] - Western blot analysis using NBP3-22624 in K562 cell lysate.")
![Immunocytochemistry/Immunofluorescence: SHB Antibody [NBP2-58223] - Staining of human cell line A549 shows localization to nucleoplasm & cytosol.](http://images.novusbio.com/fullsize/SHB-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58223-img0001.jpg "Immunocytochemistry/Immunofluorescence: SHB Antibody [NBP2-58223] - Staining of human cell line A549 shows localization to nucleoplasm & cytosol.")
![Immunohistochemistry: SHB Antibody [NBP2-30557] - Staining of human small intestine shows moderate cytoplasmic and membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/SHB-Antibody-Immunohistochemistry-NBP2-30557-img0001.jpg "Immunohistochemistry: SHB Antibody [NBP2-30557] - Staining of human small intestine shows moderate cytoplasmic and membranous positivity in glandular cells.")
![Western Blot: SHB Antibody [NBP1-58355] - HepG2 cell lysate, concentration 0.2-1 ug/ml.](http://images.novusbio.com/fullsize/SHB-Antibody-Western-Blot-NBP1-58355-img0002.jpg "Western Blot: SHB Antibody [NBP1-58355] - HepG2 cell lysate, concentration 0.2-1 ug/ml.")
![Flow Cytometry: SHB Antibody (JE57-12) [NBP3-32948] - Flow cytometric analysis of A549 cells labeling SHB.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32948, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32948_rabbit-shb-mab-je57-12-29202416372183.jpg "Flow Cytometry: SHB Antibody (JE57-12) [NBP3-32948] - Flow cytometric analysis of A549 cells labeling SHB.<br/><br/> Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32948, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).")
