SFRS4 Antibody

Images

 
Western Blot: SFRS4 Antibody [NBP2-04144] - J-Lat cells treated with sh vectors targeting SR proteins were induced with Prostratin over 24 hours. Cells were harvested for protein using RIPA. Blots for SRSF4, GFP, HIV ...read more
Immunohistochemistry-Paraffin: SFRS4 Antibody [NBP2-04144] - Sections of human lung carcinoma. Antibody: Affinity purified rabbit anti- SRp75 used at a dilution of 1:1000 (1 ug/mL). Detection: DAB. Counterstain: ...read more
Western Blot: SFRS4 Antibody [NBP2-04144] - CEM-T4 cell lysate. Western blot image submitted by a verified customer review.
Immunoprecipitation: SFRS4 Antibody [NBP2-04144] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

SFRS4 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 400 and 450 of human Pre-mRNA-Splicing Factor SRP75 using the numbering given in entry NP_005617.2 (Gene ID 6429).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SRSF4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500 to 1:2000
  • Immunohistochemistry-Paraffin 1:500 to 1:2000
  • Immunoprecipitation 2-10 ug/ml lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. SFRS4 antibody validated for WB from a verified customer review.
Reviewed Applications
Read 3 Reviews rated 4.7
using
NBP2-04144 in the following applications:

Publications
Read Publications using
NBP2-04144 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SFRS4 Antibody

  • Pre-mRNA-splicing factor SRP75
  • serine/arginine-rich splicing factor 4
  • SFRS4
  • Splicing factor, arginine/serine-rich 4SRP001LB
  • SRP75SR splicing factor 4

Background

SFRS4 - splicing factor, arginine/serine-rich 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SFRS4 Antibody (NBP2-04144)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(2)
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Filter By Species
Human
(2)
All Species

Reviews for SFRS4 Antibody (NBP2-04144) (3) 4.73

Average Rating: 4.7
(Based on 3 reviews)
We have 3 reviews tested in 2 species: Human, Mouse.

Reviews using NBP2-04144:
Filter by Applications
WB
(2)
IP
(1)
All Applications
Filter by Species
Human
(2)
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot SFRS4 NBP2-04144
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4
reviewed by:
Verified Customer
WB Human 07/24/2020
View

Summary

ApplicationWestern Blot
Sample TestedCell lysate from Jurkat cells grown in RPMI medium,Hela whole cell lysate,Human primary macrophages
SpeciesHuman
LotA1

Comments

CommentsThe J-LAt cell line used had an inducibe HIV construct that when induced would produce GFP as well as HIV proteins such as p24 (GAG). The sh vector was used to knock down the protein.
Western Blot SFRS4 NBP2-04144
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5
reviewed by:
Verified Customer
WB Human 06/09/2018
View

Summary

ApplicationWestern Blot
Sample TestedHeLa Cell lysate
SpeciesHuman
LotA1
Immunoprecipitation SFRS4 NBP2-04144
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5
reviewed by:
Verified Customer
IP Mouse 11/10/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedP19 cell line (nucleus and cytoplasm)
SpeciesMouse

Product General Protocols

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Video Protocols

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FAQs for SFRS4 Antibody (NBP2-04144) (0)

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Recent Reviews

4.7
5
2
4
1
3
0
2
0
1
0

Verified Customer
07/24/2020
Application: WB
Species: Human

Verified Customer
06/09/2018
Application: WB
Species: Human

Verified Customer
11/10/2015
Application: IP
Species: Mouse

Bioinformatics

Gene Symbol SRSF4
Uniprot