SFPQ Antibody

Western Blot: SFPQ Antibody [NBP2-20328] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 5% SDS PAGE gel, diluted at 1:10000.

Immunohistochemistry-Paraffin: SFPQ Antibody [NBP2-20328] - Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu, Mu, Rt, Bv, Pm
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SFPQ Antibody Summary

• Description: Novus Biologicals Rabbit SFPQ Antibody (NBP2-20328) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human SFPQ. The exact sequence is proprietary.
• Localization: Nucleus matrix
• Predicted Species: Mouse (99%), Rat (99%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SFPQ
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 76 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chimpanzee (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for SFPQ Antibody

• 100 kDa DNA-pairing protein
• DNA-binding p52/p100 complex, 100 kDa subunit
• hPOMp100
• polypyrimidine tract binding protein associated
• polypyrimidine tract-binding protein-associated splicing factor
• Polypyrimidine tract-binding protein-associated-splicing factor
• PSFPOMP100
• PTB-associated splicing factor
• PTB-associated-splicing factor
• splicing factor proline/glutamine rich (polypyrimidine tract binding proteinassociated)
• splicing factor proline/glutamine rich (polypyrimidine tract-bindingprotein-associated)
• splicing factor proline/glutamine-rich
• splicing factor, proline- and glutamine-rich

Background

PSF/SFPQ is a multifunctional protein know to participate in a variety of nuclear processes and may function to link transcription and pre-mRNA processing. PSF/SFPQ bears an RNA recognition motif (RRM) further indicating its role in post-transcriptional processes. PSF has been shown to associate with p54nrb/NonO to modulate androgen receptor-mediated transcription. Additionally, it has recently been show to associate with XRN2 and p54nrb/NonO to facilitate pre-mRNA 3' processing and transcription termination. Alternate names for PSF/SFPQ include polypyrimidine tract-binding protein-associated-splicing factor, PTB-associated-splicing factor, splicing factor proline-and glutamine-rich, and POMP100.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: SFPQ