SFPQ Antibody

Images

 
Western Blot: SFPQ Antibody [NB100-61045] - Detection of Human and Mouse SFPQ on HeLa whole cell lysate using NB100-61045. SFPQ was also immunoprecipitated by rabbit anti-SFPQ antibodies NB100-61043 and NB100-61044.
Immunocytochemistry/ Immunofluorescence: SFPQ Antibody [NB100-61045] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly ...read more
Immunohistochemistry: SFPQ Antibody [NB100-61045] - Sample: FFPE section of human prostate carcinoma (left) and mouse CT26 colon carcinoma (right). Antibody: Affinity purified rabbit anti- PSF/SFPQ used at a dilution of ...read more
Immunohistochemistry-Paraffin: SFPQ Antibody [NB100-61045] - FFPE section of human breast carcinoma. Antibody used at a dilution of 1:100.
Immunohistochemistry-Paraffin: SFPQ Antibody [NB100-61045] - FFPE section of human colon carcinoma. Antibody used at a dilution of 1:250.
Immunohistochemistry-Paraffin: SFPQ Antibody [NB100-61045] - FFPE section of mouse renal cell carcinoma. Antibody used at a dilution of 1:250.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

SFPQ Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 657 and 707 of human Polypyrimidine tract-binding protein-associated-splicing factor (Splicing factor, proline- and glutamine-rich) using the numbering given in entry NP_005057.1 (
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SFPQ
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:250-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
Publications
Read Publication using
NB100-61045 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SFPQ Antibody

  • 100 kDa DNA-pairing protein
  • DNA-binding p52/p100 complex, 100 kDa subunit
  • hPOMp100
  • polypyrimidine tract binding protein associated
  • polypyrimidine tract-binding protein-associated splicing factor
  • Polypyrimidine tract-binding protein-associated-splicing factor
  • PSFPOMP100
  • PTB-associated splicing factor
  • PTB-associated-splicing factor
  • splicing factor proline/glutamine rich (polypyrimidine tract binding proteinassociated)
  • splicing factor proline/glutamine rich (polypyrimidine tract-bindingprotein-associated)
  • splicing factor proline/glutamine-rich
  • splicing factor, proline- and glutamine-rich

Background

PSF/SFPQ is a multifunctional protein know to participate in a variety of nuclear processes and may function to link transcription and pre-mRNA processing. PSF/SFPQ bears an RNA recognition motif (RRM) further indicating its role in post-transcriptional processes. PSF has been shown to associate with p54nrb/NonO to modulate androgen receptor-mediated transcription. Additionally, it has recently been show to associate with XRN2 and p54nrb/NonO to facilitate pre-mRNA 3' processing and transcription termination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SFPQ Antibody (NB100-61045)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol SFPQ
Entrez
Uniprot