SFPQ Antibody

Western Blot: SFPQ Antibody [NB100-61043] - Detection of Human PSF/SFPQ on HeLa whole cell lysate (5,15, and 50 microgram) and 293T cell lysate (50 microgram) using NB100-61043. PSF/SFPQ was also immunoprecipitated by rabbit anti-PSF/SFPQ antibodies NB100-61044 and NB100-61045.

Immunohistochemistry: SFPQ Antibody [NB100-61043] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- PSF/SFPQ used at a dilution of 1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

SFPQ Antibody Summary

• Description: Novus Biologicals Rabbit SFPQ Antibody (NB100-61043) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human Polypyrimidine tract-binding protein-associated-splicing factor (Splicing factor, proline- and glutaminerich) using the numbering given in entry NP_005057.1 (GeneID 6421).
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SFPQ
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Immunohistochemistry 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SFPQ Antibody

• 100 kDa DNA-pairing protein
• DNA-binding p52/p100 complex, 100 kDa subunit
• hPOMp100
• polypyrimidine tract binding protein associated
• polypyrimidine tract-binding protein-associated splicing factor
• Polypyrimidine tract-binding protein-associated-splicing factor
• PSFPOMP100
• PTB-associated splicing factor
• PTB-associated-splicing factor
• splicing factor proline/glutamine rich (polypyrimidine tract binding proteinassociated)
• splicing factor proline/glutamine rich (polypyrimidine tract-bindingprotein-associated)
• splicing factor proline/glutamine-rich
• splicing factor, proline- and glutamine-rich

Background

PSF/SFPQ is a multifunctional protein know to participate in a variety of nuclear processes and may function to link transcription and pre-mRNA processing. PSF/SFPQ bears an RNA recognition motif (RRM) further indicating its role in post-transcriptional processes. PSF has been shown to associate with p54nrb/NonO to modulate androgen receptor-mediated transcription. Additionally, it has recently been show to associate with XRN2 and p54nrb/NonO to facilitate pre-mRNA 3' processing and transcription termination.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SFPQ
• Entrez:
  • 6421(Human)
• Uniprot:
  • P23246(Human)