SEZ6L Antibody

Western blot shows lysates of human hippocampal tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human SEZ6L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5598) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SEZ6L at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SEZ6L Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human SEZ6L
  Leu29-Ala958
  Accession # Q9BYH1
• Specificity: Detects human SEZ6L in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant mouse (rm) BSRP-B is observed and less than 2% cross-reactivity with rmBSRP-A and rmBSRP-C is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SEZ6L
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SEZ6L Antibody

• Acig1
• AIG1
• Aig1L
• BSRP-B
• seizure related 6 homolog (mouse)-like
• seizure related gene 6-like
• SEZ6L

Background

SEZ6L (Seizure 6-Like protein; also BSRP-B in rodent) is a 200 kDa member of the SEZ6 family of proteins. It is expressed in the plasma membrane and ER of CNS neurons and lung bronchiolar epithelium, and likely regulates PKC alpha activation. Mature human SEZ6L is a 996 amino acid (aa) type I transmembrane protein. It contains a 930 aa extracellular domain (ECD) (aa 29-958) plus a 45 aa cytoplasmic tail. The ECD shows three CUB domains and five short consensus repeats (SCRs). There are multiple isoforms possible for SEZ6L. One shows a deletion of aa 933-943, and a second shows the same deletion coupled to a Thr substitution for aa 803-867. A third isoform contains a Leu substitution for aa 867-943. An alternative start site is reported at Met228. This start site may occur individually, or be accompanied by a deletion of aa 932-944, the same deletion of aa 932-944 coupled to a Thr substitution for aa 803-867, or a duel deletion of aa 869-945 and 1016-1024. Over aa 29-958, human BSRP-B is 80% aa identical to mouse BSRP-B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SEZ6L Antibody (AF5598)(1)

Publication using AF5598

• M Marino, L Zhou, MY Rincon, Z Callaerts-, J Verhaert, J Wahis, E Creemers, L Yshii, K Wierda, T Saito, C Marneffe, I Voytyuk, Y Wouters, M Dewilde, SI Duqué, C Vincke, Y Levites, TE Golde, TC Saido, S Muylderman, A Liston, B De Stroope, MG Holt AAV-mediated delivery of an anti-BACE1 VHH alleviates pathology in an Alzheimer's disease model Embo Molecular Medicine, 2022-03-30;14(4):e09824. 2022-03-30 [PMID: 35352880] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SEZ6L
• Uniprot:
  • Q9BYH1(Human)