CHFR Antibody (1H3-A12)

Images

 
Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - CHFR monoclonal antibody (M01), clone 1H3-A12. Analysis of CHFR expression in 293.
Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 2 ug/ml.
Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Western Blot analysis of CHFR expression in A-431 ( Cat # L015V1 ).
Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi ( Cat # H00055743-R01V ) (Lane 2) or non-transfected control (Lane ...read more
Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M01), clone 1H3-A12.Lane 1: CHFR transfected lysate (Predicted MW: 72.1 ...read more
Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue.
Sandwich ELISA: CHFR Antibody (1H3-A12) [H00055743-M01] - Detection limit for recombinant GST tagged CHFR is approximately 0.1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
1H3-A12
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CHFR Antibody (1H3-A12) Summary

Immunogen
CHFR (AAH12072, 1 a.a. ~ 653 a.a) full length recombinant protein with GST tag.MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFDTSGAGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLD
Specificity
CHFR - checkpoint with forkhead and ring finger domains
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CHFR
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), RNAi validation, and ELISA.
Reviewed Applications
Read 1 Review rated 4
using
H00055743-M01 in the following applications:

Publications
Read Publications using H00055743-M01.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CHFR Antibody (1H3-A12)

  • Checkpoint with forkhead and RING finger domains protein
  • checkpoint with forkhead and ring finger domains
  • E3 ubiquitin-protein ligase CHFR
  • EC 6.3.2.-
  • FLJ10796
  • FLJ33629
  • RING finger protein 196
  • RNF196RNF116

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CHFR Antibody (H00055743-M01)(7)


Showing Publications 1 - 7 of 7.
Publications using H00055743-M01 Applications Species
Kashima L, Idogawa M, Mita H et al. CHFR regulates the mitotic checkpoint by targeting PARP-1 for ubiquitination and degradation. J Biol Chem. 2012 Feb 15 [PMID: 22337872]
Fujita A, Yamamoto H, Imamura M et al. Expression level of the mitotic checkpoint protein and G2-M cell cycle regulators and prognosis in gastrointestinal stromal tumors in the stomach. Virchows Arch. 2011 Dec 22 [PMID: 22190007]
Hu SL, Huang DB, Sun YB et al. Pathobiologic implications of methylation and expression status of Runx3 and CHFR genes in gastric cancer. Med Oncol. 2010 Mar 19 [PMID: 20300977]
Gao YJ, Xin Y, Zhang JJ et al. Mechanism and pathobiologic implications of CHFR promoter methylation in gastric carcinoma. World J Gastroenterol 14(32):5000-7. 2008 Aug 28. [PMID: 18763281]
Privette LM, Weier JF, Nguyen HN et al. Loss of CHFR in human mammary epithelial cells causes genomic instability by disrupting the mitotic spindle assembly checkpoint. Neoplasia;10(7):643-52. 2008 Jul. [PMID: 18592005]
Privette LM, Gonzalez ME, Ding L et al. Altered Expression of the Early Mitotic Checkpoint Protein, CHFR, in Breast Cancers: Implications for Tumor Suppression. Cancer Res;67(13):6064-74. 2007 Jul 1. [PMID: 17596595]
Milne AN, Sitarz R, Carvalho R et al. Molecular analysis of primary gastric cancer, corresponding xenografts, 2 novel gastric carcinoma cell lines reveals novel alterations in gastric carcinogenesis. Hum Pathol;38(6):903-13. 2007 Jun. [PMID: 17376510]

Review for CHFR Antibody (H00055743-M01) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: IHC-P.

Reviews using H00055743-M01:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Motofumi Tanaka
IHC-P Human 05/12/2010
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedHuman colon cancer tissue
SpeciesHuman

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:100, Primary Ab Incubation Time: 60 minutes

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Motofumi Tanaka
05/12/2010
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol CHFR
Entrez
OMIM
Uniprot