CHFR Antibody (1H3-A12) - Azide and BSA Free

Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - CHFR monoclonal antibody (M01), clone 1H3-A12. Analysis of CHFR expression in 293.

Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 2 ug/ml.

Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Western Blot analysis of CHFR expression in A-431 ( Cat # L015V1 ).

Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR over-expressed 293 cell line, cotransfected with CHFR Validated Chimera RNAi ( Cat # H00055743-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CHFR monoclonal antibody (M01), clone 1H3-A12 (Cat # H00055743-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: CHFR Antibody (1H3-A12) [H00055743-M01] - Analysis of CHFR expression in transfected 293T cell line by CHFR monoclonal antibody (M01), clone 1H3-A12.Lane 1: CHFR transfected lysate (Predicted MW: 72.1 KDa).Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: CHFR Antibody (1H3-A12) [H00055743-M01] - Immunoperoxidase of monoclonal antibody to CHFR on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma tissue.

Sandwich ELISA: CHFR Antibody (1H3-A12) [H00055743-M01] - Detection limit for recombinant GST tagged CHFR is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC, KD
• Clone: 1H3-A12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CHFR Antibody (1H3-A12) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse CHFR Antibody (1H3-A12) - Azide and BSA Free (H00055743-M01) is a monoclonal antibody validated for use in IHC, WB and ELISA. Anti-CHFR Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: CHFR (AAH12072.1, 1 a.a. ~ 652 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFDTSGAGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRVEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN
• Specificity: CHFR - checkpoint with forkhead and ring finger domains
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CHFR
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin), RNAi validation, and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CHFR Antibody (1H3-A12) - Azide and BSA Free

• Checkpoint with forkhead and RING finger domains protein
• checkpoint with forkhead and ring finger domains
• E3 ubiquitin-protein ligase CHFR
• EC 6.3.2.-
• FLJ10796
• FLJ33629
• RING finger protein 196
• RNF196RNF116

Background

E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CHFR Antibody (H00055743-M01)(9)

Publications using H00055743-M01

• Woo SR, Lee HJ, Oh SJ et al. Stabilization of HDAC1 via TCL1-pAKT-CHFR axis is a key element for NANOG-mediated multi-resistance and stem-like phenotype in immune-edited tumor cells. Biochem Biophys Res Commun 2018-07-29 [PMID: 30060952]
• Zhang D, Xu XL, Li F et al. Upregulation of the checkpoint protein CHFR is associated with tumor suppression in pancreatic cancers. Oncol Lett 2017-10-20 [PMID: 29344247]
• Kashima L, Idogawa M, Mita H et al. CHFR regulates the mitotic checkpoint by targeting PARP-1 for ubiquitination and degradation. J Biol Chem. 2012-02-15 [PMID: 22337872]
• Fujita A, Yamamoto H, Imamura M et al. Expression level of the mitotic checkpoint protein and G2-M cell cycle regulators and prognosis in gastrointestinal stromal tumors in the stomach. Virchows Arch. 2011-12-22 [PMID: 22190007]
• Hu SL, Huang DB, Sun YB et al. Pathobiologic implications of methylation and expression status of Runx3 and CHFR genes in gastric cancer. Med Oncol. 2010-03-19 [PMID: 20300977]
• Gao YJ, Xin Y, Zhang JJ et al. Mechanism and pathobiologic implications of CHFR promoter methylation in gastric carcinoma. World J Gastroenterol 14(32):5000-7. 2008-08-28 [PMID: 18763281]
• Privette LM, Weier JF, Nguyen HN et al. Loss of CHFR in human mammary epithelial cells causes genomic instability by disrupting the mitotic spindle assembly checkpoint. Neoplasia;10(7):643-52. 2008-07-01 [PMID: 18592005]
• Privette LM, Gonzalez ME, Ding L et al. Altered Expression of the Early Mitotic Checkpoint Protein, CHFR, in Breast Cancers: Implications for Tumor Suppression. Cancer Res;67(13):6064-74. 2007-07-01 [PMID: 17596595]
• Milne AN, Weterman MA et al. Molecular analysis of primary gastric cancer, corresponding xenografts, 2 novel gastric carcinoma cell lines reveals novel alterations in gastric carcinogenesis. Hum Pathol;38(6):903-13. 2007-06-01 [PMID: 17376510]

Review for CHFR Antibody (H00055743-M01) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Motofumi Tanaka IHC-P Human 05/12/2010

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Human colon cancer tissue
• Species: Human

Bioinformatics

• Gene Symbol: CHFR
• Entrez:
  • 55743(Human)
• OMIM:
  • 605209(Human)
• Uniprot:
  • AAH12072(Human)