sFRP-4 Antibody - BSA Free

Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human endometrium, kidney, liver and placenta using Anti-SFRP4 antibody (A) shows similar protein distribution across tissues to independent antibody (B).

Western Blot: sFRP-4 Antibody [NBP2-38632] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma

Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human endometrium shows moderate secreted positivity in cells in endometrial stroma.

Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human kidney shows no positivity in cells in tubules as expected.

Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human liver shows no positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: sFRP-4 Antibody [NBP2-38632] - Staining of human placenta shows no positivity in trophoblastic cells as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

sFRP-4 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: PTLATYLSKNYSYVIHAKIKAVQRSGCNEVTTVVDVKEIFKSSSPIPRTQVPLITNSSCQCPHILPHQDVLIMCYEWRSRMMLLENCLVEKWRDQLSKRSIQWEERLQEQRRTVQDKKKTAGRTSRSNPPKPKGKPPAPKPASPK
• Predicted Species: Mouse (92%), Rat (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SFRP4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  sFRP-4 Protein (NBP2-38632PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for sFRP-4 Antibody - BSA Free

• DDC-4
• Frizzled protein, human endometrium
• FRP-4
• FRP-AP
• frpHE
• FRPHEMGC26498
• FrzB2
• secreted frizzled-related protein 4
• secreted frizzled-related protein 4; secreted frizzled-related protein 4
• sFRP4
• sFRP-4

Background

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for sFRP-4 Antibody (NBP2-38632) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 09/30/2019

Summary

• Application: Western Blot
• Sample Tested: Whole cell lysate
• Species: Human
• Lot: R60484

Bioinformatics

• Gene Symbol: SFRP4