SEZ6L Antibody

SEZ6L was detected in perfusion fixed frozen sections of mouse brain (hippocampus) using Sheep Anti-Mouse SEZ6L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4804) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell bodies and processes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

SEZ6L Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse SEZ6L
  Glu32-Ser889
  Accession # AAH65117
• Specificity: Detects mouse SEZ6L in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) BSRP-A and rmBSRP-C is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SEZ6L
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SEZ6L Antibody

• Acig1
• AIG1
• Aig1L
• BSRP-B
• seizure related 6 homolog (mouse)-like
• seizure related gene 6-like
• SEZ6L

Background

SEZ6L (Seizure 6-Like protein; also BSRP-B/Brain-specific receptor-like protein B) is a 200-210 kDa member of the SEZ6 family of proteins. It is expressed in the plasma membrane and ER of CNS neurons, particularly cerebellar interneurons plus Purkinje and granule cells. SEZ6L likely regulates Ca++ availability and thus impacts PKC alpha activation and mGluR1 signaling. Mature mouse SEZ6L is a 932 amino acid (aa) type I transmembrane protein. It contains an 866 aa extracellular domain (ECD) (aa 32-897) and a 45 aa cytoplasmic tail. The ECD shows three CUB domains and five short consensus repeats (SCRs). There is one 190-200 kDa splice variant termed BSRP-B that contains a Phe substitution for aa 807-872 of the fifth SCR domain. Over aa 32-889, mouse SEZ6L shares 80% and 97% aa identity with human and rat SEZ6L, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SEZ6L Antibody (AF4804)(4)

Publications using AF4804

• Martina Pigoni, Hung‐En Hsia, Jana Hartmann, Jasenka Rudan Njavro, Merav D Shmueli, Stephan A Müller et al. Seizure protein 6 controls glycosylation and trafficking of kainate receptor subunits GluK2 and GluK3 The EMBO Journal 2020-08-03 [PMID: 32567721]
• Ubadah Sabbagh, Aboozar Monavarfeshani, Kaiwen Su, Masoud Zabet‐Moghadam, James Cole, Eric Carnival et al. Distribution and development of molecularly distinct perineuronal nets in visual thalamus Journal of Neurochemistry 2018-12-01 [PMID: 30326149]
• Ubadah Sabbagh, Aboozar Monavarfeshani, Kaiwen Su, Masoud Zabet‐Moghadam, James Cole, Eric Carnival et al. Distribution and development of molecularly distinct perineuronal nets in visual thalamus Journal of Neurochemistry 2018-12-01 [PMID: 30326149] (Western Blot, Mouse, Transgenic Mouse)
• Martina Pigoni, Johanna Wanngren, Peer-Hendrik Kuhn, Kathryn M. Munro, Jenny M. Gunnersen, Hiroshi Takeshima et al. Seizure protein 6 and its homolog seizure 6-like protein are physiological substrates of BACE1 in neurons Molecular Neurodegeneration 2016-12-01 [PMID: 27716410]

Bioinformatics

• Gene Symbol: SEZ6L
• Uniprot:
  • AAH65117()