Serpin G1/C1 Inhibitor Antibody (350507) [Unconjugated]

Western blot shows lysates of human lung tissue and human ovary tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Serpin G1/C1 Inhibitor Monoclonal Antibody (Catalog # MAB2488) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin G1/C1 Inhibitor at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, IP
• Clone: 350507
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Serpin G1/C1 Inhibitor Antibody (350507) [Unconjugated] Summary

• Immunogen: Human plasma-derived Serpin G1/C1 Inhibitor
• Specificity: Detects human Serpin G1/C1 Inhibitor in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Serpin A1, A3, A4, A5, C1, D1, E2, or F1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SERPING1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin G1/C1 Inhibitor Antibody (350507) [Unconjugated]

• C1 Inhibitor
• C1IN
• C1INH
• C1NH
• HAE1
• HAE2
• Serpin G1
• SERPING1 serpin peptidase inhibitor, clade G (C1 inhibitor), member 1

Background

As a member of the serpin superfamily of serine protease inhibitors, Serpin G1/C1 inhibitor is the physiological inhibitor of activated C1r and C1s, two serine proteases involved in the classical complement pathway. In addition, it inhibits plasma kallikrein and coagulation factor XIIa, two serine proteases involved in the processing of kininogen to release bradykinin. Therefore, it plays an important role in regulating activation of both the complement and contact systems (1). Serpin G1 deficiency results in hereditary angioedema, which is characterized by recurrent episodes of localized angioedema of the skin, gastrointestinal mucosa or upper respiratory mucosa (2). The deduced amino acid sequence of human Serpin G1 precursor consists of 500 residues with a signal peptide. The mature protein of 478 amino acid residues is heavily glycosylated (1).

1. Davis, A.E. III et al. (1993) Methods Enzymol. 223:97.
2. Davis, A.E. III (2004) Drug News Perspect. 17:439.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin G1/C1 Inhibitor Antibody (MAB2488)(1)

Publication using MAB2488

• Zhang Y, Vernooij F, Ibrahim I, Ooi S, Gijsberts C, Schoneveld A, Sen K, den Ruijter H, Timmers L, Richards A, Jong C, Mazlan I, Wang J, Lam C, de Kleijn D Extracellular Vesicle Proteins Associated with Systemic Vascular Events Correlate with Heart Failure: An Observational Study in a Dyspnoea Cohort. PLoS ONE, 2016-01-28;11(1):e0148073. 2016-01-28 [PMID: 26820481] (ELISA Development, Human)

Bioinformatics

• Gene Symbol: SERPING1