Recombinant Human Serpin F1/PEDF Protein

Product Discontinued

Recombinant Human Serpin F1/PEDF Protein Summary

• Description: Recombinant protein containing 400 amino acids for Human Serpin F1/PEDF
  
  

  Source:E. coli
  Amino Acid Sequence:(P36955)

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: SERPINF1
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 44.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: Sterile filtered and lyophilized from a concentrated solution containing 20 mM PBS (pH 7.4), 150 mM NaCl
• Concentration: Lyoph
• Purity: >95%, by SDS-PAGE
• Reconstitution Instructions: Centrifuge vial prior to opening. Reconstitute in sterile dH2O to a concentration of 0.1 -1 mg/ml and let the lyophilized pellet dissolve completely. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long

Alternate Names for Recombinant Human Serpin F1/PEDF Protein

• Cell proliferation-inducing gene 35 protein
• EPC-1
• EPC-1PIG35
• PEDF
• PEDFpigment epithelium-derived factor
• pigment epithelium derived factor), member 1
• proliferation-inducing protein 35
• serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin
• Serpin F1
• serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1

Background

PEDF is a noninhibitory serpin with neurotrophic, anti-angiogenic, and anti-tumorigenic properties. PEDF is a 50,000 dalton glycoprotein created and secreted in many tissues all the way through the body. A key component of the anti-angiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. Additionally, PEDF is capable to inhibit the activity of angiogenic factors such as VEGF and FGF-2. The recognition of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. The unique range of PEDF activities associate it as a potential therapeutic agent for the treatment of vasculature related neurodegenerative diseases such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR). PEDF in addition has the potential to be functional in the treatment of various angiogenesis-related diseases including a number of cancers. Recombinant human PEDF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 400 amino acids and having a molecular mass of 44.5 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SERPINF1