Serpin F1/PEDF Antibody (1C4)

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Western Blot: Serpin F1/PEDF Antibody (1C4) [NBP1-04331] - A549 cell lysate was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PEDF antibody (1:1000). Protein was visualized using a goat ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-04331
    • Availability
      Product Discontinued

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Serpin F1/PEDF Antibody (1C4) Summary

Immunogen
Recombinant human PEDF (19-418 aa) purified from E. coli (NP_002606).
Specificity
Anti-human PEDF mAb, clone 1C4, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PEDF protein.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
SERPINF1
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:100-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Serpin F1/PEDF Antibody (1C4)

  • Cell proliferation-inducing gene 35 protein
  • EPC-1
  • EPC-1PIG35
  • PEDF
  • PEDFpigment epithelium-derived factor
  • pigment epithelium derived factor), member 1
  • proliferation-inducing protein 35
  • serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin
  • Serpin F1
  • serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1

Background

Pigment epithelium-derived factor (PEDF) is a 50kDa glycoprotein and member of the serpin superfamily without any activity of serine protease. PEDF is an angiogenesis inhibitor with neurotrophic properties, balances angiogenesis in the eye and blocks tumor progression. That is, in neuronal cell, PEDF is promoting cell survival and differentiation, however, when endothelial cells are exposed to PEDF, their cell migration and proliferation are inhibited, and they undergo apoptosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Serpin F1/PEDF Products

Bioinformatics Tool for Serpin F1/PEDF Antibody (NBP1-04331)

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Diseases for Serpin F1/PEDF Antibody (NBP1-04331)

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PTMs for Serpin F1/PEDF Antibody (NBP1-04331)

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Bioinformatics

Gene Symbol SERPINF1
Entrez
Uniprot