Serpin E2/PN1 Antibody (OTI2C9)

Western Blot: Serpin E2/PN1 Antibody (2C9) [NBP2-01984] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SERPINE2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SERPINE2.

Immunohistochemistry-Paraffin: Serpin E2/PN1 Antibody (2C9) [NBP2-01984] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-SERPINE2 mouse monoclonal antibody.

Flow Cytometry: Serpin E2/PN1 Antibody (2C9) [NBP2-01984] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SERPINE2 antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: Serpin E2/PN1 Antibody (2C9) [NBP2-01984] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SERPINE2 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Serpin E2/PN1 Antibody (2C9) [NBP2-01984] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SERPINE2 mouse monoclonal antibody.

• Reactivity: Hu, Mu
• Applications: WB, Flow, IHC
• Clone: OTI2C9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.76 mg/ml

Serpin E2/PN1 Antibody (OTI2C9) Summary

• Immunogen: Full length human recombinant protein of human SERPINE2(NP_006207) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SERPINE2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Theoretical MW: 41.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.76 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Serpin E2/PN1 Antibody (OTI2C9)

• GDN
• GdNPF
• GDNserine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 2
• glia-derived nexin
• glial-derived neurite promoting factor
• Peptidase inhibitor 7
• PI-7
• PI7DKFZp686A13110
• PN-1
• PN1nexin
• PNI
• Protease nexin 1
• Protease nexin I
• Proteinase Nexin 1
• Serpin E2
• serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitortype 1), member 2

Background

Serine protease inhibitor with activity toward thrombin, trypsin, and urokinase. Promotes neurite extension by inhibiting thrombin. Binds heparin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SERPINE2
• Entrez:
  • 5270(Human)