Serpin B3/SCCA1 Antibody (886524) [Unconjugated]

Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Serpin B3/SCCA1 Monoclonal Antibody (Catalog # MAB6528) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B3/SCCA1 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Serpin B3/SCCA1 was detected in immersion fixed paraffin-embedded sections of human squamous cell carcinoma using Mouse Anti-Human Serpin B3/SCCA1 Monoclonal Antibody (Catalog # MAB6528) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 886524
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Serpin B3/SCCA1 Antibody (886524) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin B3/SCCA1
  Asn2-Pro390
  Accession # P29508
• Specificity: Detects human Serpin B3/SCCA1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: SERPINB3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Serpin B3/SCCA1 Antibody (886524) [Unconjugated]

• HsT1196
• Protein T4-A
• SCC
• SCCA
• SCCA1
• SCCA1SCCA-1
• SCCA-PD
• serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 3
• Serpin B3
• serpin peptidase inhibitor, clade B (ovalbumin), member 3
• Squamous cell carcinoma antigen 1
• T4-A

Background

Serpin B3, also known as Squamous Cell Carcinoma Antigen-1 (SCCA1), is a member of the serpin superfamily of serine protease inhibitors (1). Serpins primarily inhibit serine proteases and some are known to inhibit caspases and papain-like cysteine proteases (1). Serpin B3 belongs to the subgroup ovalbumin-related serpins which are involved in the regulation of apoptosis, inflammation, angiogenesis and embryogenesis (2). Serpin B3 was initially isolated from human cervical squamous cell tissue. It is a tumor marker for squamous cell carcinomas in various areas including the cervix, head, neck and lung (3). Unlike typical serpins, Serpin B3 shows inhibitory activity against papain-like lysosomal cysteine proteases Cathepsin K, L, and S, while lacking inhibitory activity against serine proteases (4).

1. Law, R. et al. (2006) Genome Biol. 7:216.
2. Benarafa, C. and Remold-O’Donnell, E. (2005) Proc. Natl. Acad. Sci. 102:11367.
3. Higgens, W.J. et al. (2010) J. Biol. Chem. 285:3722.
4. Schick, C. et al. (1998) Biochemistry. 37:5258.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Serpin B3/SCCA1 Antibody (MAB6528)(1)

Publication using MAB6528

• Ohara, Y;Tang, W;Liu, H;Yang, S;Dorsey, TH;Cawley, H;Moreno, P;Chari, R;Guest, MR;Azizian, A;Gaedcke, J;Ghadimi, M;Hanna, N;Ambs, S;Hussain, SP; SERPINB3-MYC axis induces the basal-like/squamous subtype and enhances disease progression in pancreatic cancer Cell reports 2023-11-18 [PMID: 37980563] (Western Blot, Human)

Bioinformatics

• Gene Symbol: SERPINB3
• Uniprot:
  • P29508()