Separase Antibody (XJ11-4D7)

Images

 
Immunocytochemistry/ Immunofluorescence: Separase Antibody (XJ11-4D7) [NB100-330] - Overlay [blue] of centrosomal staining in HeLa cells. Centrosomal staining of separase [yellow], using NB 100-330, and gamma tubulin ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clone
XJ11-4D7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Separase Antibody (XJ11-4D7) Summary

Immunogen
Maltose-Binding Protein fusion of C-terminal fragment of human Separase (amino acids 1866-1996). [UniProt# Q14674]
Localization
Cytoplasmic and Nuclear
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
ESPL1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Western Blot
Application Notes
Use in Western Blot reported in scientific literature (PMID:17626243).This Separase (XJ11-4D7) antibody is useful for Immunocytochemistry/Immunofluorescence. In ICC/IF, this antibody recognizes endogenous Separase in paraformaldehyde-fixated preparations of human cells.
Publications
Read Publications using
NB100-330 in the following applications:

  • WB
    4 publications

Reactivity Notes

Human. Use in Mouse reported in scientific literature (PMID:17626243).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for Separase Antibody (XJ11-4D7)

  • Caspase-like protein ESPL1
  • EC 3.4.22.49
  • ESP1extra spindle poles like 1
  • extra spindle pole bodies homolog 1 (S. cerevisiae)
  • extra spindle poles like 1 (S. cerevisiae)
  • Extra spindle poles-like 1 protein
  • FLJ46492
  • KIAA0165separin
  • Separase

Background

In normal cellular proliferation, the maintenance of constant centriole numbers is strongly associated with cell cycle process and the disengagement of mother-daugther centriole (a requirement for duplication of centriole) is facilitated by separase (ESPL1, a cysteine endopeptidase) mediated proteolytic cleavage of cohesin, a "glue" protein complex which is also essential to sister-chromatide cohesion. Separase is a caspase-like protease which plays a key role in chromosome segregation by cleaving SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle phases, separase is kept inactivated, and its proteolytic activity is tightly regulated by multiple inhibitory mechanisms combining securin binding, CyclinB1/Cdk1 mediated serine residue phosphorylation (pSer1226), PP2A binding and autocatalytic cleavage. Ectopic activation of separase proteolytic activity results in premature sister-chromatide separation/centriole disengagement and separase overexpression has been shown to induce aneuploidy and carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Separase Antibody (NB100-330)(4)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol ESPL1
Entrez
Uniprot