Semaphorin 4G Antibody

Western blot shows lysates of mouse brain (cortex) tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse Semaphorin 4G Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6504) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Semaphorin 4G at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1

Semaphorin 4G was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Mouse Semaphorin 4G Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6504) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counter-stained with hematoxylin (blue). Specific staining was localized to developing brain (cortex) and connective tissue. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Semaphorin 4G Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4G
  Val18-Met673
  Accession # Q9WUH7
• Specificity: Detects mouse Semaphorin 4G in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human Semaphorin 4G is observed, and less than 1% cross-reactivity with recombinant mouse (rm) Semaphorin 4B, rmSemaphorin 4C, and rmSemaphorin 4F is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SEMA4G
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 4G Antibody

• KIAA1619FLJ20590
• MGC102867
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4G
• SEMA4G
• Semaphorin 4G
• semaphorin-4G

Background

Semaphorin 4G (Sema4G) is a 92 kDa (predicted), class IV member of the semaphorin family of proteins. It is expressed by select cell types, including oligodendroglia and Purkinje neurons, and likely acts as a chemorepellent by binding to B-type plexins. Mature mouse Sema4G is a type I transmembrane protein that is 820 amino acids (aa) in length. It contains a 656 aa extracellular region (aa 18-673) that is characterized by one Sema domain (aa 35-503), a PSI region (aa 505‑556), and an Ig-like C2-type domain (aa 565-648). The 143 aa cytoplasmic region contains a poly-proline segment and a Ser phosphorylation site. Over aa 18‑673, mouse Sema4G shares 91% and 97% aa identity with human and rat Sema4G, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEMA4G
• Uniprot:
  • Q9WUH7()