Semaphorin 4G Antibody [Alexa Fluor® 405]

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 405

Semaphorin 4G Antibody [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4G
  Val18-Met673
  Accession # Q9WUH7
• Specificity: Detects mouse Semaphorin 4G in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human Semaphorin 4G is observed, and less than 1% cross-reactivity with recombinant mouse (rm) Semaphorin 4B, rmSemaphori
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 4G Antibody [Alexa Fluor® 405]

• KIAA1619FLJ20590
• MGC102867
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4G
• SEMA4G
• Semaphorin 4G
• semaphorin-4G

Background

Semaphorin 4G (Sema4G) is a 92 kDa (predicted), class IV member of the semaphorin family of proteins. It is expressed by select cell types, including oligodendroglia and Purkinje neurons, and likely acts as a chemorepellent by binding to B-type plexins. Mature mouse Sema4G is a type I transmembrane protein that is 820 amino acids (aa) in length. It contains a 656 aa extracellular region (aa 18-673) that is characterized by one Sema domain (aa 35-503), a PSI region (aa 505‑556), and an Ig-like C2-type domain (aa 565-648). The 143 aa cytoplasmic region contains a poly-proline segment and a Ser phosphorylation site. Over aa 18‑673, mouse Sema4G shares 91% and 97% aa identity with human and rat Sema4G, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.