Semaphorin 4D/CD100 Antibody (758726) [Phycoerythrin]

Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human Semaphorin 4D/CD100 PE-conjugated Monoclonal Antibody (Catalog # FAB74701P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 758726
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Semaphorin 4D/CD100 Antibody (758726) [Phycoerythrin] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Semaphorin 4D/CD100
  Met22-Arg734
  Accession # Q92854
• Specificity: Detects human Semaphorin 4D/CD100 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 4B, 4C, 4G, or recombinant mouse Semaphorin 4D is observed. In Western blots, no cross-reactivity with rhSemaphorin 4A, 6A, or 6C is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SEMA4D
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 4D/CD100 Antibody (758726) [Phycoerythrin]

• A8
• BB18
• CD100 antigen
• CD100
• CD100M-sema-G
• COLL-4
• FLJ33485
• FLJ34282
• FLJ39737
• FLJ46484
• immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D
• MGC169138
• MGC169141
• M-sema-G
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D
• SEMA4D
• SEMAJ
• Semaphorin 4D
• semaphorin-4D

Background

Semaphorin 4D (Sema4D)/CD100, previously Sem J, G or C-like 2, is a widely expressed 150 kDa type I transmembrane glycoprotein of the Class 4 Semaphorin family with activity in the immune and nervous systems. The 861 amino acid (aa) Semaphorin 4D contains a 710 aa extracellular domain (ECD) with a sema domain and an immunoglobulin-like domain. Within the ECD region used as an immunogen, mouse Semaphorin 4D shares 87% and 93% aa identity with human and rat Semaphorin 4D, respectively. A soluble, active 120 kDa form is produced proteolytically upon activation of B and T cells. Semaphorin 4D binds CD72 on B and dendritic cells and Plexin B1 elsewhere. In developing neurons, Semaphorin 4D assists in guidance. Semaphorin 4D is proangiogenic when expressed by tumor-associated macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Semaphorin 4D/CD100 Antibody (FAB74701P)(2)

Publications using FAB74701P

• Y Li, L Qin, Q Bai, J Zhang, R Chen, K Song CD100 modulates cytotoxicity of CD8+ T cells in patients with acute myocardial infarction BMC immunology, 2021-02-16;22(1):13. 2021-02-16 [PMID: 33593275] (Flow Cytometry, Human)
• BJ Li, Y He, Y Zhang, YH Guo, Y Zhou, PX Zhang, W Wang, JR Zhao, JG Li, WZ Zuo, C Fan, ZS Jia Interferon-?-induced CD100 on na�ve CD8(+) T cells enhances antiviral responses to hepatitis C infection through CD72 signal transduction J. Int. Med. Res, 2017-01-12;45(1):89-100. 2017-01-12 [PMID: 28222623] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: SEMA4D
• Uniprot:
  • Q92854()