Semaphorin 4D/CD100 Antibody (547412)

Mouse splenocytes were stained with Mouse Semaphorin 4D/CD100 Monoclonal Antibody (Catalog # MAB52351, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B).

• Reactivity: Mu
• Applications: Flow, CyTOF-ready
• Clone: 547412
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Semaphorin 4D/CD100 Antibody (547412) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4D/CD100
  Phe24-Thr657
  Accession # NP_038688
• Specificity: Detects mouse Semaphorin 4D/CD100 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Semaphorin 3A, 3B, 3C, 3D, 3E, 3F, 4A, 4B, 4C, 4D, 4G, 5A, 5B, 6A, 6B, 6C, 6D, or 7A is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: SEMA4D
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Semaphorin 4D/CD100 Antibody (547412)

• A8
• BB18
• CD100 antigen
• CD100
• CD100M-sema-G
• COLL-4
• FLJ33485
• FLJ34282
• FLJ39737
• FLJ46484
• immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D
• MGC169138
• MGC169141
• M-sema-G
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D
• SEMA4D
• SEMAJ
• Semaphorin 4D
• semaphorin-4D

Background

Semaphorin 4D (Sema4D/CD100, previously sem J, G or C-like 2) is a widely expressed 150 kDa type I transmembrane glycoprotein of the Class 4 Semaphorin family with activity in the immune and nervous systems. The 861 amino acid (aa) Sema4D contains a 710 aa extracellular domain (ECD) with a sema domain and an immunoglobulin-like domain. Within the ECD region used as an immunogen, mouse Sema4D shares 87% and 93% aa identity with human and rat Sema4D, respectively. A soluble, active 120 kDa form is produced proteolytically upon activation of B and T cells. Sema4D binds Plexin-B1, but also CD72 on B and dendritic cells. In developing neurons, Sema-4D assists in guidance. Sema4D is proangiogenic when expressed by tumor-associated macrophages.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEMA4D
• Uniprot:
  • NP_038688()