Semaphorin 4D/CD100 Antibody (21E8) - BSA Free

ELISA: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] - The Binding Activity of Mouse Semaphorin 4D/CD100 with Semaphorin 4D/CD100 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Mouse Semaphorin 4D/CD100 at 2 ug/mL can bind Semaphorin 4D/CD100 Antibody, the EC50 is 98.14-174.8 ng/mL.

ELISA: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] - The Binding Activity of Human Semaphorin 4D/CD100 with Semaphorin 4D/CD100 Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human Semaphorin 4D/CD100 at 2 ug/mL can bind Semaphorin 4D/CD100 Antibody, the EC50 is 1.082-4.805 ng/mL.

Flow Cytometry: Semaphorin 4D/CD100 Antibody (21E8) [NBP3-26330] - Untransfected HEK293T cells (green line) and transfected Human SEMA4D HEK293T stable cells (red line) were stained with anti-SEMA4D antibody (2ug/1*10^6 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody and analyzed with flow cytometry.

• Reactivity: Hu
• Applications: ELISA, Flow
• Clone: 21E8
• Clonality: Monoclonal
• Host: Human
• Conjugate: Unconjugated
• Format: BSA Free

Semaphorin 4D/CD100 Antibody (21E8) - BSA Free Summary

• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: Recombinant Human Semaphorin 4D/CD100 protein [UniProt Q92854]
• Isotype: IgG4 (S228P)
• Clonality: Monoclonal
• Host: Human
• Gene: SEMA4D
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry 1:50-1:200

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol, 0.01M PBS, PH 7.4
• Preservative: 0.03% Proclin 300
• Purity: Affinity purified

Alternate Names for Semaphorin 4D/CD100 Antibody (21E8) - BSA Free

• A8
• BB18
• CD100 antigen
• CD100
• CD100M-sema-G
• COLL-4
• FLJ33485
• FLJ34282
• FLJ39737
• FLJ46484
• immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D
• MGC169138
• MGC169141
• M-sema-G
• sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D
• SEMA4D
• SEMAJ
• Semaphorin 4D
• semaphorin-4D

Background

Semaphorins are neuronal chemorepellants that direct pioneering neurons during nervous system development. CD100, a 150 kDa homodimer, is a novel semaphorin that induces B cells to aggregate and improves their viability in vitro. CD100 modifies CD40-CD40L B cell signaling by augmenting B cell aggregation and survival and down regulating CD23 expression. CD72 is a lymphocyte receptor for the class IV semaphorin CD100, which is involved in regulating B cell signaling. CD100 is expressed on resting and PHA stimulated T cells. The protein is weakly expressed on NK cells, EBV transformed B cells, monocytes and tumor/peripheral blood T cell lines, and at higher density on activated T cells and B cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEMA4D