Semaphorin 3E Antibody - BSA Free

Western Blot: Semaphorin 3E Antibody [NBP3-23381] - Western blot analysis of rat PC12 cells (lanes 1 & 2) and human PC-3 cells (lanes 3 & 4). The blots were probed with anti-Semaphorin 3E (N-terminal region) antibody in absence (lanes 1 & 3) or presence of Semaphorin 3E (N-terminal region) blocking peptide (lanes 2 & 4).

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Semaphorin 3E Antibody - BSA Free Summary

• Description: Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
• Immunogen: Semaphorin 3E (N-terminal region) synthetic peptide (coupled to carrier protein) corresponds to amino acids in the N-terminal region of human Semaphorin 3E. This sequence is well conserved in rat, mouse, and chicken Semaphorin 3E, and has low homology to other semaphorin family members. [UniProt# O15041]
• Specificity: The antibody detects 87 and 61 kDa bands corresponding to the full-length and furin-proccessed forms of Semaphorin 3E in human PC-3 and rat PC12 cells.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SEMA3E
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for Semaphorin 3E Antibody - BSA Free

• (semaphorin) 3E
• coll-5
• KIAA0331M-SEMAH
• sema domain, immunoglobulin domain (Ig), short basic domain, secreted
• Sema3E
• SEMAH
• SEMAHM-SemaK
• Semaphorin 3E
• semaphorin-3E

Background

Semaphorins, such as Semaphorin 3E, are characterized by a conserved domain of about 500 amino acids. These proteins are involved in embryonic development, and some behave as neural guidance molecules.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SEMA3E