Plexin D1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of JAR human choriocarcinoma cell line, IMR‑32 human neuroblastoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Goat ...read more
Plexin D1 was detected in immersion fixed paraffin-embedded sections of human melanoma using Goat Anti-Human Plexin D1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4160) at 3 µg/mL overnight at 4 °C. ...read more
Human peripheral blood monocytes were stained with Goat Anti-Human Plexin D1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4160, filled histogram) or control antibody (Catalog # AB‑108‑C, open ...read more
Recombinant Human Semaphorin 3E (Catalog # 3239-S3) inhibits proliferation in the HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Plexin D1 mediated inhibition elicited by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, B/N, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

Plexin D1 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Plexin D1
Leu47-Ala1271
Accession # Q9Y4D7
Specificity
Detects human Plexin D1 in direct ELISAs and Western blots.
Details of Functionality
ActivityAssaywCitation not found None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PLXND1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 3-15 ug/mL
  • CyTOF-ready
  • Neutralization 0.5-2 ug/mL
Publications
Read Publications using
AF4160 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Plexin D1 Antibody [Unconjugated]

  • KIAA0620MGC75353
  • PLEXD1
  • Plexin D1
  • plexin-D1
  • PLXND1

Background

Plexin D1 is a type I transmembrane glycoprotein that is the prototype of the plexin D subfamily of semaphorin receptors (1, 2). Human Plexin D1 contains a 46 amino acid (aa) signal sequence, a 1225 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 633 aa cytoplasmic domain that includes features common to other plexins (1). The human Plexin D1 ECD shares 89% identity with mouse Plexin D1, and ~84-92% aa identity based on incomplete sequences of rat, bovine, porcine and canine Plexin D1. It contains a sema domain, two plexin-semaphorin-integrin (PSI) or Met-related sequence (MRS) cysteine-rich motifs, and three glycine/proline-rich IPT/TIG domains which are immunoglobulin-like domains found in plexins, transcription factors, and the scatter factor receptors Met and Ron (1, 2). Isoforms of 1787 and 1747 aa have been sequenced; these contain a 178 aa N-terminal deletion with or without a longer alternate C-terminus (3). Like other Sema/plexin interactions, Plexin D1 interacts with Sema3C or Sema4A via neuropilins. Interaction with Sema3E, however, is direct (4). Plexin D1/Sema3E interaction mediates vascular guidance during development or angiogenesis; deletion of either molecule results in similar, profound cardiac abnormalities (4, 5). Plexin D1 is also expressed in lymphocytes, osteoblasts, the neural crest and the central nervous system during development (2, 6). In the brain, the presence of neuropilin can change Plexin D1/Sema3E interaction from an attractive to a repulsive signal (7, 8). Plexin D1 directs migration of thymocytes to the thymic medulla, probably through repulsion of Sema3E (9). Endothelial cell Plexin D1 binding to Sema4A can oppose VEGF and suppresses tumor angiogenesis, and expression of Sema3E correlates inversely with tumor metastasis, indicating that Plexin D1 is anti-metastatic in the presence of its ligands (10, 11).

  1. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
  2. Van Der Zwaag, B. et al. (2002) Dev. Dyn. 225:336.
  3. Entrez protein Accession # Q9Y4D7, EAW79239, EAW79240.
  4. Gu, C. et al. (2005) Science 307:265.
  5. Gitler, A.D. et al. (2004) Developmental Cell 7:107.
  6. Zhang, Y. et al. (2009) Dev. Biol. 325:82.
  7. Chauvet, S. et al. (2007) Neuron 56:807.
  8. Pecho-Vrieseling, E. et al. (2009) Nature 459:842.
  9. Choi, Y.I. et al. (2008) Immunity 29:888.
  10. Toyofuku, T. et al. (2007) EMBO J. 26:1373.
  11. Roodink, I. et al. (2008) Am. J. Pathol. 173:1873.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF3239
Species: Hu, Mu
Applications: WB, IHC, ICC
AF566
Species: Mu, Rt
Applications: WB, Flow, IHC, Block, CyTOF-ready
NBP1-58310
Species: Hu, Mu, Rt, Bv, Ca, Eq, GP, Rb, Sh, Ze
Applications: WB, IHC, IHC-P
AF567
Species: Mu, Rt
Applications: WB, Simple Western, IHC, Block
NBP2-49588
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF5486
Species: Hu, Mu, Rt
Applications: WB, Simple Western, Flow, CyTOF-ready, ICC
NBP1-88478
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-81480
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF5329
Species: Hu
Applications: WB, IHC
NB100-2218
Species: Hu, Mu, Rt, Bv, Ca, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NB100-56161
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
NBP1-98542
Species: Hu, Mu, Rt, Rb
Applications: WB
AF3749
Species: Hu
Applications: WB, IHC
NBP1-92684
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
NBP2-25160
Species: Hu, Mu, Rt, Po, Bv, Ca, Eq
Applications: WB, ICC/IF, IHC
AF4160
Species: Hu
Applications: WB, Flow, IHC, B/N, CyTOF-ready

Publications for Plexin D1 Antibody (AF4160)(6)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: Flow Cytometry, ICC, IHC, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(1)
ICC
(1)
IHC
(1)
IHC-P
(1)
Western Blot
(2)
All Applications
Filter By Species
Human
(4)
Mouse
(2)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF4160 Applications Species
K Burk, E Mire, A Bellon, M Hocine, J Guillot, F Moraes, Y Yoshida, M Simons, S Chauvet, F Mann Post-endocytic sorting of Plexin-D1 controls signal transduction and development of axonal and vascular circuits Nat Commun, 2017;8(0):14508. 2017 [PMID: 28224988] (ICC, Mouse) ICC Mouse
Hesam Movassagh Chemorepellent Semaphorin 3E Negatively Regulates Neutrophil Migration In Vitro and In Vivo J. Immunol, 2016;0(0):. 2016 [PMID: 27913633] (Flow Cytometry, Human) Flow Cytometry Human
Semaphorin 3G Provides a Repulsive Guidance Cue to Lymphatic Endothelial Cells via Neuropilin-2/PlexinD1 Cell Rep, 2016;17(9):2299-2311. 2016 [PMID: 27880905] (Western Blot, Human) Western Blot Human
Tata A, Stoppel D, Hong S, Ben-Zvi A, Xie T, Gu C An image-based RNAi screen identifies SH3BP1 as a key effector of Semaphorin 3E-PlexinD1 signaling. J Cell Biol, 2014;205(4):573-90. 2014 [PMID: 24841563] (IHC, Human) IHC Human
Lu, Kuan-Min, Evans, Sylvia M, Hirano, Shinji, Liu, Fu-Chin Dual role for Islet-1 in promoting striatonigral and repressing striatopallidal genetic programs to specify striatonigral cell identity. Proc Natl Acad Sci U S A, 2014;111(1):E168-77. 2014 [PMID: 24351932] (IHC-P, Mouse) IHC-P Mouse
Casazza A, Finisguerra V, Capparuccia L Sema3E-Plexin D1 signaling drives human cancer cell invasiveness and metastatic spreading in mice. J. Clin. Invest., 2010;120(8):2684-98. 2010 [PMID: 20664171] (Western Blot, Human) Western Blot Human

Reviews for Plexin D1 Antibody (AF4160) (0)

There are no reviews for Plexin D1 Antibody (AF4160). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Plexin D1 Antibody (AF4160) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 488 FAB4160G
Biotin BAF4160
Phycoerythrin FAB4160P

Additional Plexin D1 Products

Bioinformatics Tool for Plexin D1 Antibody (AF4160)

Discover related pathways, diseases and genes to Plexin D1 Antibody (AF4160). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Plexin D1 Antibody (AF4160)

Discover more about diseases related to Plexin D1 Antibody (AF4160).
 

Pathways for Plexin D1 Antibody (AF4160)

View related products by pathway.

PTMs for Plexin D1 Antibody (AF4160)

Learn more about PTMs related to Plexin D1 Antibody (AF4160).

Blogs on Plexin D1

There are no specific blogs for Plexin D1, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Plexin D1 Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol PLXND1
Uniprot