Secretagogin Antibody (778518) [Unconjugated]

Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line and human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Secretagogin Monoclonal Antibody (Catalog # MAB4878) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Secretagogin at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Secretagogin was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Secretagogin Monoclonal Antibody (Catalog # MAB4878) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of beta TC‑6 mouse beta cell insulinoma cell line and human pancreas tissue, loaded at 0.5 mg/mL. A specific band was detected for Secretagogin at approximately 37 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Secretagogin Monoclonal Antibody (Catalog # MAB4878). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clone: 778518
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Secretagogin Antibody (778518) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Secretagogin
  Asn164-Pro276
  Accession # O76038
• Specificity: Detects human Secretagogin in ELISAs and Western Blots. Detects mouse Secretagogin in Western Blots
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SCGN
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Secretagogin Antibody (778518) [Unconjugated]

• CALBL
• DJ501N12.8
• SCGN
• SECRET
• Secretagogin
• secretagogin, EF-hand calcium binding protein
• SECRETsecretagogin
• SEGN
• Setagin

Background

Secretagogin (SCGN) is a 32 kDa member of the EF-hand family of Ca-binding proteins. It is expressed in select neurons, as well as neuroendocrine cells and pancreatic islets. Human Secretagogin is 276 amino acids (aa) in length and contains six 35 aa EF-hand domains (aa 12‑276). There is one alternative splice variant that shows a 22 aa substitution for aa 28‑276, and which demonstrates no Ca-binding ability. Secretagogin is known to circulate, presumably released during cell apoptosis. Over aa 164‑276, human Secretagogin is more than 97% aa identical to mouse, porcine and canine Secretagogin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Secretagogin Antibody (MAB4878) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IHC-P Human 04/05/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Pancreas tissue
• Species: Human
• Lot: 778518

Comments

• Comments: Incubated tissue overnight at 4C at 15 µg/mL

Bioinformatics

• Gene Symbol: SCGN
• Uniprot:
  • O76038(Human)