SDHB Antibody (CL0347) - BSA Free

Western Blot: SDHB Antibody (CL0347) [NBP2-52920] - Lane 1: Marker [kDa], Lane 2: Human cell line RT-4

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920] - Staining of human ventricular cancer shows strong granular cytoplasmic immunoreactivity in tumor cells.

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920] - Staining of human breast cancer shows strong granular cytoplasmic immunoreactivity in tumor cells.

Immunohistochemistry: SDHB Antibody (CL0347) [NBP2-52920] - Staining of human colorectal cancer shows strong granular cytoplasmic positivity in tumor cells.

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920] - Staining of human kidney shows strong granular positivity in the cytoplasm of renal tubules cells.

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920] - Staining of human liver cancer shows strong positivity in tumor cells.

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920] - Staining of human liver shows strong granular cytoplasmic immunoreactivity in hepatocytes.

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920] - Staining of human rectum shows strong granular cytoplasmic immunoreactivity in glandular cells.

Immunohistochemistry-Paraffin: SDHB Antibody (CL0347) [NBP2-52920] - Staining of human testis shows strong granular cytoplasmic positivity in seminiferous tubules.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL0347
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

SDHB Antibody (CL0347) - BSA Free Summary

• Description: Novus Biologicals Mouse SDHB Antibody (CL0347) - BSA Free (NBP2-52920) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from P21912, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (97%), Rat (97%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SDHB
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  SDHB Recombinant Protein Antigen (NBP2-52920PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for SDHB Antibody (CL0347) - BSA Free

• EC 1.3.5.1
• FLJ92337
• IP
• Iron-sulfur subunit of complex II
• PGL4
• SDH
• SDH1
• SDH2
• SDHIP
• succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
• succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

Background

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for SDHB Antibody (NBP2-52920) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 11/04/2023

Summary

• Application: Western Blot
• Sample Tested: glioblastoma cell lysates
• Species: Human
• Lot: 02694

Bioinformatics

• Gene Symbol: SDHB