SCUBE2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SCUBE2 Antibody - BSA Free [NBP1-77186] - Immunofluorescence of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 ug/mL.

Immunocytochemistry/ Immunofluorescence: SCUBE2 Antibody - BSA Free [NBP1-77186] - Immunocytochemistry of SCUBE2 in Daudi cells with SCUBE2 antibody at 20 ug/mL.

Western Blot: SCUBE2 Antibody - BSA Free [NBP1-77186] - Western blot analysis of SCUBE2 in Daudi cell lysate with SCUBE2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SCUBE2 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide near the center of human SCUBE2. The immunogen is located within amino acids 480 - 530 of SCUBE2. Amino Acid Squence: CSPLRNKQQKSNDSAFGD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SCUBE2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunofluorescence 20 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  SCUBE2 Peptide (NBP1-77186PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SCUBE2 Antibody - BSA Free

• Cegb1
• CEGF1
• CEGP1
• FLJ16792
• FLJ35234
• MGC133057
• Protein CEGP1
• signal peptide, CUB and EGF-like domain-containing protein 2
• signal peptide, CUB domain, EGF-like 2

Background

SCUBE2 is a member of a family of secreted glycoproteins that contain N-terminal EGF-like repeats and C-terminal cysteine-rich motifs and CUB domain and are expressed in embryonic neuroectoderm and vascular epithelium. SCUBE2 is a close homolog to SCUBE1 and can form a heterodimeric complex with SCUBE1. SCUBE2 is thought to participate in the Hedgehog signaling pathway by specifically interacting with Sonic Hedgehog (SHH) and the receptor PTCH1, enhancing the SHH signaling activity in the raft microdomains of the plasma membrane, and may thus play important roles in organ development and tumor progression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCUBE2
• Uniprot:
  • NP_066025(Human)