SCUBE1 Antibody

Images

 
Western Blot: SCUBE1 Antibody [NBP1-77185] - Daudi cell lysate with SCUBE1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry/ Immunofluorescence: SCUBE1 Antibody [NBP1-77185] - Daudi cells with SCUBE1 antibody at 20 ug/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SCUBE1 Antibody Summary

Immunogen
Antibody was raised against a 14 amino acid synthetic peptide near the center of human SCUBE1. The immunogen is located within amino acids 460 - 510 of SCUBE1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SCUBE1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Immunofluorescence
Control Peptide
SCUBE1 Peptide (NBP1-77185PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SCUBE1 Antibody

  • signal peptide, CUB and EGF-like domain-containing protein 1
  • signal peptide, CUB domain, EGF-like 1
  • signal peptide-CUB domain-EGF-related 1

Background

SCUBE1 is an EGF-related protein with a CUB (C1s-like) domain that is expressed primarily in the developing gonad, nervous system, somites, surface ectoderm and limb buds. The N-terminal EGF-like repeats of SCUBE1 participate in the formation of adhesions in a Ca2+-dependent fashion. In contrast, the C-terminal cysteine-rich motifs and CUB domain can directly bind and antagonize bone morphogenic protein activity; genetic ablation of this region resulted in brain malformation in mice embryos, suggesting SCUBE1 plays a role in brain morphogenesis. SCUBE1 has also been implicated in early craniofacial development in mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SCUBE1 Antibody (NBP1-77185) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol SCUBE1
Uniprot