SCUBE1 Antibody - BSA Free

Western Blot: SCUBE1 Antibody [NBP1-77185] - Daudi cell lysate with SCUBE1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry/ Immunofluorescence: SCUBE1 Antibody - BSA Free [NBP1-77185] - Immunocytochemistry of SCUBE1 in Daudi cells with SCUBE1 antibody at 20 u/mL.

Immunocytochemistry/ Immunofluorescence: SCUBE1 Antibody - BSA Free [NBP1-77185] - Immunofluorescence of SCUBE1 in Daudi cells with SCUBE1 antibody at 20 ug/mL.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SCUBE1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SCUBE1 Antibody - BSA Free (NBP1-77185) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 14 amino acid synthetic peptide near the center of human SCUBE1. The immunogen is located within amino acids 460 - 510 of SCUBE1. Amino Acid Squence: CSDAPTTPIKQKAR
• Predicted Species: Mouse (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SCUBE1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  SCUBE1 Peptide (NBP1-77185PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SCUBE1 Antibody - BSA Free

• signal peptide, CUB and EGF-like domain-containing protein 1
• signal peptide, CUB domain, EGF-like 1
• signal peptide-CUB domain-EGF-related 1

Background

SCUBE1 is an EGF-related protein with a CUB (C1s-like) domain that is expressed primarily in the developing gonad, nervous system, somites, surface ectoderm and limb buds. The N-terminal EGF-like repeats of SCUBE1 participate in the formation of adhesions in a Ca2+-dependent fashion. In contrast, the C-terminal cysteine-rich motifs and CUB domain can directly bind and antagonize bone morphogenic protein activity; genetic ablation of this region resulted in brain malformation in mice embryos, suggesting SCUBE1 plays a role in brain morphogenesis. SCUBE1 has also been implicated in early craniofacial development in mice.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SCUBE1
• Uniprot:
  • NP_766638(Human)