SCL/Tal1 Antibody

Staining of human placenta shows weak cytoplasmic positivity in trophoblastic cells.

Staining of human colon shows moderate cytoplasmic positivity in glandular cells.

Staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells.

Analysis in human cell line NTERA-2.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

SCL/Tal1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids:DAIMSPEEELDGYEPEPLGKRPAVLPLLELVGESGNNTSTDGSLPSTPPPAEEEEDELYRQSLEIISRYLREQATGAKDTKPMGRSGATSRKALETLRRVGDG
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TAL1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for SCL/Tal1 Antibody

• BHLHA17
• bHLHa17tal-1
• Class A basic helix-loop-helix protein 17
• SCL
• SCLT-cell acute lymphocytic leukemia protein 1
• Stem Cell Protein
• Tal1
• TAL-1
• T-cell acute lymphocytic leukemia 1
• T-cell leukemia/lymphoma protein 5
• TCL5
• TCL5tal-1 product

Background

Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SCL/Tal1 Antibody (NBP1-90005)(3)

Publications using NBP1-90005

• Guantes R, Rastrojo A, Neves R et al. Global variability in gene expression and alternative splicing is modulated by mitochondrial content. Genome Res 2015-05-01 [PMID: 25800673]
• Nemati F, de Montrion C, Lang G et al. Targeting Bcl-2/Bcl-XL Induces Antitumor Activity in Uveal Melanoma Patient-Derived Xenografts. PLoS One 2014-01-01 [PMID: 24454684]
• Aries IM, Hansen BR, Koch T et al. The synergism of MCL1 and glycolysis on pediatric acute lymphoblastic leukemia cell survival and prednisolone resistance. Haematologica 2013-12-01 [PMID: 24142999]

Bioinformatics

• Gene Symbol: TAL1