SCGF/CLEC11a Antibody [Unconjugated]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

SCGF/CLEC11a Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse SCGF/CLEC11a
  Ala22-Phe328
  Accession # O88200
• Specificity: Detects mouse SCGF/CLEC11a in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human SCGF is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: Clec11a
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SCGF/CLEC11a Antibody [Unconjugated]

• CLEC11a
• CLECSF3
• LSLCL
• P47
• SCGF

Background

SCGF, also named CLEC11a, is a 47 kDa, 328 amino acid, secreted glycoprotein member of the C-type lectin superfamily. Produced by CD33⁺ stem cells and resting chondrocytes, SCGF acts in combination with other factors to promote erythroid, myeloid and endothelial cell production. Over the region used as immunogen, mouse SCGF is 92% and 83% aa identical to the corresponding rat and human protein sequences, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SCGF/CLEC11a Antibody (AF3729)(7)

Publications using AF3729

• Aoyuan Fan, Genbin Wu, Jianfang Wang, Laiya Lu, Jingyi Wang, Hanjing Wei et al. Inhibition of fibroblast activation protein ameliorates cartilage matrix degradation and osteoarthritis progression Bone Research 1/2/2023 [PMID: 36588124]
• Stephen Gadomski, Claire Fielding, Andrés García-García, Claudia Korn, Chrysa Kapeni, Sadaf Ashraf et al. A cholinergic neuroskeletal interface promotes bone formation during postnatal growth and exercise Cell Stem Cell 4/7/2022 [PMID: 35276096]
• Zhang J, Cohen A, Shen B et al. The effect of parathyroid hormone on osteogenesis is mediated partly by osteolectin Proceedings of the National Academy of Sciences of the United States of America 6/22/2021 [PMID: 34140410]
• H Wei, Y Xu, Y Wang, L Xu, C Mo, L Li, B Shen, Y Sun, P Cheng, L Yang, Y Pang, A Qin, Y Cao, SJ Morrison, R Yue Identification of Fibroblast Activation Protein as an Osteogenic Suppressor and Anti-osteoporosis Drug Target Cell Rep, 2020-10-13;33(2):108252. 2020-10-13 [PMID: 33053358] (IHC, Mouse)
• Y Hu, Y Zhang, CY Ni, CY Chen, SS Rao, H Yin, J Huang, YJ Tan, ZX Wang, J Cao, ZZ Liu, PL Xie, B Wu, J Luo, H Xie Human umbilical cord mesenchymal stromal cells-derived extracellular vesicles exert potent bone protective effects by CLEC11A-mediated regulation of bone metabolism Theranostics, 2020-01-16;10(5):2293-2308. 2020-01-16 [PMID: 32089743] (Western Blot, Mouse)
• P Ramalingam, MG Poulos, E Lazzari, MC Gutkin, D Lopez, CC Kloss, MJ Crowley, L Katsnelson, AG Freire, MB Greenblatt, CY Park, JM Butler Chronic activation of endothelial MAPK disrupts hematopoiesis via NFKB dependent inflammatory stress reversible by SCGF Nat Commun, 2020-02-03;11(1):666. 2020-02-03 [PMID: 32015345] (ELISA (detection), Mouse)
• Bo Shen, Kristy Vardy, Payton Hughes, Alpaslan Tasdogan, Zhiyu Zhao, Rui Yue et al. Integrin alpha11 is an Osteolectin receptor and is required for the maintenance of adult skeletal bone mass eLife 1/11/2019 [PMID: 30632962]

Bioinformatics

• Gene Symbol: Clec11a
• Uniprot:
  • O88200()