SCF/c-kit Ligand Antibody

Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 15 ug of lysates per lane.Antibodies: SCF (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/Immunofluorescence: SCF/c-kit Ligand Antibody [NBP1-77030] - SCF in Human Brain tissue with SCF antibody at 20 ug/mL.

Immunohistochemistry-Paraffin: SCF/c-kit Ligand Antibody [NBP1-77030] - Mouse brain tissue with SCF antibody at 2.5 ug/ml.

Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Analysis of SCF in A431 cell lysate with SCF antibody at (A) 1 and (B) 2 ug/mL.

Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Analysis of protein expression level of SCF detected by anti-SCF antibody. GSMCs were treated with C-type natriuretic peptide (CNP) at different doses for 48hr. CNP(10^-7mol/L and 10^-6mol/L) significantly decreased SCF protein expression level as compared to the control group.

Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - ISOFORMS: Human SCF has 3 isoforms, including isoform 1 (273aa, 30.9kD), isoform 2 (245aa, 27.9kD) and isoform 3 (238aa, 26.7kD). Mouse SCF has 2 isoforms, including isoform 1 (273aa, 30.6kD) and isoform 2 (245aa, 27.6kD). Rat SCF also has 2 isoforms, including isoform 1 (273aa, 30.7kD) and isoform 2 (245aa, 27.7kD). This antibody can detect human and possibly detect mouse and rat.

Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - WB analysis showed protein expression level of SCF detected by anti-SCF antibody in gastric smooth muscle was significantly decreased in STZ-induced diabetic mice as compared to the control.

Western Blot: SCF/c-kit Ligand Antibody [NBP1-77030] - Loading: 30 ng of human SCF recombinant protein per lane.Antibodies: SCF (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.Observed at around 20kD.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

SCF/c-kit Ligand Antibody Summary

• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide from near the center of human SCF. The immunogen is located within amino acids 100 - 150 of SCF. Amino Acid Squence: KENSSKDLKKSFKSPEPR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KITLG
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-2 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
• Theoretical MW: 23, 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  SCF/c-kit Ligand Peptide (NBP1-77030PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SCF/c-kit Ligand Antibody

• c-kit Ligand
• DCUA
• DFNA69
• DKFZp686F2250
• familial progressive hyperpigmentation 2
• FPH2
• FPHH
• KIT ligand
• Kitl
• KITLG
• KL-1
• Mast cell growth factor
• MGF
• MGFSHEP7
• SCF
• SCFStem cell factor
• SFc-Kit ligand
• SHEP7
• SLF
• steel factor

Background

Stem cell factor (SCF) is the ligand of the c-Kit oncogene and is expressed by various structural and inflammatory cells in the airways. Binding of SCF by the c-Kit receptor leads to homodimerization of the receptor and the activation of signalling pathways such as PI-3, PLC-gamma, Jak/STAT, and MAP kinase pathways. SCF expression leads to the induction of mast cell survival and the expression and release of histamine, pro-inflammatory cytokines and chemokines. The inhibition of the SCF/c-Kit pathway leads to a decrease in histamine levels, mast cell and eosinophil infiltration, IL-4 production and airway hyperresponsiveness, suggesting this pathway may be a useful therapeutic target in inflammatory diseases such as asthma. At least two isoforms of SCF are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SCF/c-kit Ligand Antibody (NBP1-77030)(3)

Publications using NBP1-77030

• Jensen BM, Metcalfe DD, Gilfillan AM. Targeting kit activation: a potential therapeutic approach in the treatment of allergic inflammation. Inflamm. Allergy Drug Targets; 6:57-62. 2007 [PMID: 17352689]
• Reber L, Da Silva CA, Frossard N. Stem cell factor and its receptor c-Kit as targets for inflammatory diseases. Euro. J. Pharmacology; 533:327-40. 2006 [PMID: 16483568]
• Lukacs NW, Strieter RM, Lincoln PM et al. Stem cell factor (c-kit ligand) influences eosinophil recruitment and histamine levels in allergic airway inflammation. J. Immunol. 156:3945-51. 1996 [PMID: 8621935]

Bioinformatics

• Gene Symbol: KITLG
• Entrez:
  • 4254(Human)
  • 17311(Mouse)
  • 60427(Rat)
• Uniprot:
  • P21583()