SCF/c-kit Ligand Antibodies

455-MC) stimulates prolif-eration in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse SCF/c-kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA). The ND₅₀ is typically 0.3-0.6 µg/mL." class="big_lightbox"> 455-MC) stimulates prolif-eration in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse SCF/c-kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA). The ND₅₀ is typically 0.3-0.6 µg/mL." alt="Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC) stimulates prolif-eration in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse SCF/c-kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA). The ND₅₀ is typically 0.3-0.6 µg/mL." class="big_thumb" />

455-MC) was serially diluted and captured by Goat Anti-Mouse SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 455-MC) was serially diluted and captured by Goat Anti-Mouse SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

455-MC) was serially diluted and captured by Goat Anti-Mouse SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 455-MC) was serially diluted and captured by Goat Anti-Mouse SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

255-SC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/c-kit Ligand (10 ng/mL) is neutralized (green line) by increasing concentrations of Human SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA). The ND₅₀ is typically 0.05-0.3 µg/mL." class="big_lightbox"> 255-SC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/c-kit Ligand (10 ng/mL) is neutralized (green line) by increasing concentrations of Human SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA). The ND₅₀ is typically 0.05-0.3 µg/mL." alt="Recombinant Human SCF/c-kit Ligand (255-SC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/c-kit Ligand (10 ng/mL) is neutralized (green line) by increasing concentrations of Human SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA). The ND₅₀ is typically 0.05-0.3 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

255-SC) was serially diluted and captured by Mouse Anti-Human SCF/c‑kit Ligand Monoclonal Antibody (Catalog # MAB655) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 255-SC) was serially diluted and captured by Mouse Anti-Human SCF/c‑kit Ligand Monoclonal Antibody (Catalog # MAB655) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

255-SC) was serially diluted and captured by Mouse Anti-Human SCF/c‑kit Ligand Monoclonal Antibody (Catalog # MAB655) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 255-SC) was serially diluted and captured by Mouse Anti-Human SCF/c‑kit Ligand Monoclonal Antibody (Catalog # MAB655) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human SCF/c‑kit Ligand (Catalog # 255-SC) was serially diluted and captured by Mouse Anti-Human SCF/c‑kit Ligand Monoclonal Antibody (Catalog # MAB655) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

255-SC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/c-kit Ligand (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human SCF/c-kit Ligand Polyclonal Antibody (Catalog # AB-255-NA). The ND₅₀ is typically 0.4-2 µg/mL." class="big_lightbox"> 255-SC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/c-kit Ligand (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human SCF/c-kit Ligand Polyclonal Antibody (Catalog # AB-255-NA). The ND₅₀ is typically 0.4-2 µg/mL." alt="Recombinant Human SCF/c-kit Ligand (Catalog # 255-SC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/c-kit Ligand (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human SCF/c-kit Ligand Polyclonal Antibody (Catalog # AB-255-NA). The ND₅₀ is typically 0.4-2 µg/mL." class="big_thumb" />

455-MC) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse SCF/c-kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse SCF/ c-kit Ligand Polyclonal Antibody (Catalog # AB-455-NA). The ND₅₀ is typically 4-20 µg/mL." class="big_lightbox"> 455-MC) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse SCF/c-kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse SCF/ c-kit Ligand Polyclonal Antibody (Catalog # AB-455-NA). The ND₅₀ is typically 4-20 µg/mL." alt="Recombinant Mouse SCF/ c-kit Ligand (Catalog # 455-MC) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse SCF/c-kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse SCF/ c-kit Ligand Polyclonal Antibody (Catalog # AB-455-NA). The ND₅₀ is typically 4-20 µg/mL." class="big_thumb" />

2278-SC) stimulates pro-liferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine SCF/c-kit Ligand (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine SCF/ c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2278). The ND₅₀ is typically 0.1-0.3 µg/mL." class="big_lightbox"> 2278-SC) stimulates pro-liferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine SCF/c-kit Ligand (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine SCF/ c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2278). The ND₅₀ is typically 0.1-0.3 µg/mL." alt="Recombinant Canine SCF/c-kit Ligand (Catalog # 2278-SC) stimulates pro-liferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine SCF/c-kit Ligand (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Canine SCF/ c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2278). The ND₅₀ is typically 0.1-0.3 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF016). A specific band was detected for SCF/c-kit Ligand at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for SCF/c-kit Ligand at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of rat adipose tissue, rat liver tissue, and mouse adipose tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6998) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SCF/c-kit Ligand at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

455-MC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse SCF/c-kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse SCF/c-kit Ligand Monoclonal Antibody (Catalog # MAB455). The ND₅₀ is typically 5-30 µg/mL." class="big_lightbox"> 455-MC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse SCF/c-kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse SCF/c-kit Ligand Monoclonal Antibody (Catalog # MAB455). The ND₅₀ is typically 5-30 µg/mL." alt="Recombinant Mouse SCF/c-kit Ligand (Catalog # 455-MC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse SCF/c-kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse SCF/c-kit Ligand Monoclonal Antibody (Catalog # MAB455). The ND₅₀ is typically 5-30 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="SCF/c-kit Ligand was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) using Goat Anti-Feline SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2268) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />