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455-MC) stimulates prolif-eration in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse SCF/c-kit Ligand (25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA). The ND50 is typically 0.3-0.6 µg/mL." class="big_lightbox" target="_blank">
455-MC) was serially diluted and captured by Goat Anti-Mouse SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
455-MC) was serially diluted and captured by Goat Anti-Mouse SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-455-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
255-SC) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human SCF/c-kit Ligand (10 ng/mL) is neutralized (green line) by increasing concentrations of Human SCF/c-kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA). The ND50 is typically 0.05-0.3 µg/mL." class="big_lightbox" target="_blank">
255-SC) was serially diluted and captured by Mouse Anti-Human SCF/c‑kit Ligand Monoclonal Antibody (Catalog # MAB655) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human SCF/c‑kit Ligand Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-255-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
BT-SCF-GMP), Animal-Free (BT-SCF-AFL) and RUO (Catalog # BT-SCF) grades of Recombinant Human SCF as measured in a cell proliferation assay using TF-1 human erythroleukemic cells (orange, green, red, respectively)." class="big_lightbox" target="_blank">
Mouse SCF (Stem cell factor, steel factor, Kit ligand, C-kit-ligand) is a hematopoietic growth factor that stimulates the proliferation of bone marrow stem cells. SCF induces the proliferation of mast cells and primitive lymphoid and myeloid hematopoietic bone marrow progenitor cells in culture. SCF acts synergistically with both GM-CSF and G-CSF, IL-7 and EPO. Mouse SCF is active on human cells as well as murine cells and can be used for long term bone marrow culture.