Sca-1/Ly6 Antibody (177228) [Allophycocyanin]

Mouse splenocytes gated on hematopoietic lineage negative cells were stained with Rat Anti-Mouse CD117/c-kit PE-conjugated Monoclonal Antibody (Catalog # FAB1356P) and either (A) Rat Anti-Mouse Sca-1/Ly6 APC-conjugated Monoclonal Antibody (Catalog # FAB1226A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 177228
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Allophycocyanin

Sca-1/Ly6 Antibody (177228) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Sca‑1/Ly6 C-terminally truncated Ly-6E allele
  Leu27-Gly119
  Accession # CAA28351
• Specificity: Detects mouse Sca‑1/Ly6 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Ly6a
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sca-1/Ly6 Antibody (177228) [Allophycocyanin]

• Ly6
• Ly-6
• Ly-6A.2
• Ly-6A/E
• Ly-6E.1
• lymphocyte antigen 6 complex, locus A
• lymphocyte antigen 6A-2/6E-1
• Sca1
• Sca-1
• Stem cell antigen 1
• TAP
• T-cell-activating protein

Background

Stem Cell Antigen-1 (Sca-1) is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2, 3). This antibody is frequently used in combination with lineage depletion antibodies to identify and isolate murine HSC. Sca-1-positive HSC can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen in the adult animal (2‑7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9‑12).

1. Van de Rijn, M. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4634.
2. Spangrude, G.I. et al. (1988) Science 241:58.
3. Spangrude, G.I. et al. (1993) Blood 82:3327.
4. Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
5. Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
6. Yamamoto, Y. et al. (1996, Blood 88:445.
7. Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
8. Welm, B.E. et al. (2002) Dev. Biol. 245:42.
9. Codias, E.K. et al. (1990) J. Immunol. 144:2197.
10. Malek, T.R. et al. (1986) J. Exp. Med. 164:709.
11. Codias, E.K. et al. (1990) J. Immunol. 145:1407.
12. Flood, P.M. et al. (1990) J. Exp. Med. 172:115.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sca-1/Ly6 Antibody (FAB1226A)(2)

Publications using FAB1226A

• H Watanabe, S Goto, R Kato, S Komiyama, Y Nagaoka, T Kazama, C Yamamoto, Y Li, N Konuma, K Hagikura, T Matsumoto The neovascularization effect of dedifferentiated fat cells Sci Rep, 2020-06-08;10(1):9211. 2020-06-08 [PMID: 32514018] (Flow Cytometry, Mouse)
• Srivastava MK, Sinha P, Clements VK Myeloid-derived suppressor cells inhibit T-cell activation by depleting cystine and cysteine. Cancer Res., 2009-12-22;70(1):68-77. 2009-12-22 [PMID: 20028852] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: Ly6a
• Uniprot:
  • CAA28351()