Sca-1/Ly6 Antibody (177228) [Biotin]

Sca-1/Ly6 was detected in perfusion fixed frozen sections of mouse kidney using Mouse Sca-1/Ly6 Biotinylated Monoclonal Antibody (Catalog # BAM1226) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Sca-1/Ly6 was detected in perfusion fixed frozen sections of mouse thymus using Mouse Sca-1/Ly6 Biotinylated Monoclonal Antibody (Catalog # BAM1226) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, Flow, IHC
• Clone: 177228
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin
• Concentration: LYOPH

Sca-1/Ly6 Antibody (177228) [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Sca-1 C-terminally truncated Ly-6E allele
  Leu27-Gly119
  Accession # CAA28351
• Specificity: Detects mouse Sca-1/Ly6 in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: Ly6a
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Sca-1/Ly6 Antibody (177228) [Biotin]

• Ly6
• Ly-6
• Ly-6A.2
• Ly-6A/E
• Ly-6E.1
• lymphocyte antigen 6 complex, locus A
• lymphocyte antigen 6A-2/6E-1
• Sca1
• Sca-1
• Stem cell antigen 1
• TAP
• T-cell-activating protein

Background

Sca-1 is an 18 kDa phosphatidylositol-anchored protein that is a member of the lymphocyte antigen 6 (Ly-6) family (1). Sca-1 is encoded by the strain-specific Ly-6 E/A allelic gene. Its expression on multipotent hematopoietic stem cells (HSC) has been used as a marker of HSC in mice of both Ly-6 haplotypes (2,3). Sca-1-positive HSC can be found in the adult bone marrow, fetal liver, and mobilized peripheral blood and spleen in the adult animal (2-7). However, Sca-1 has also been discovered in several non-hematopoietic tissues (1) and can be used to enrich progenitor cell populations other than HSC (8). It is suggested that Sca-1 could be involved in regulating both B and T cell activation (9-12).

1. Van de Rijn, M. et al. (1989) Proc. Natl. Acad. Sci. USA 86:4634.
2. Spangrude, G.I. et al. (1988) Science 241:58.
3. Spangrude, G.I. et al. (1993) Blood 82:3327.
4. Morrison, S.J. et al. (1995) Proc. Natl. Acad. Sci. USA 92:10302.
5. Kawamoto, H. et al. (1997) Int. Immunol. 9:1011.
6. Yamamoto, Y. et al. (1996) Blood 88:445.
7. Morrison, S.J. et al. (1997) Proc. Natl. Acad. Sci. USA 94:1908.
8. Welm, B.E. et al. (2002) Dev. Biol. 245:42.
9. Codias, E.K. et al. (1990) J. Immunol. 144:2197.
10. Malek, T.R. et al. (1986) J. Exp. Med. 164:709.
11. Codias, E.K. et al. (1990) J. Immunol. 145:1407.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Sca-1/Ly6 Antibody (BAM1226)(7)

Publications using BAM1226

• Hansen, SL;Larsen, HL;Pikkupeura, LM;Maciag, G;Guiu, J;Müller, I;Clement, DL;Mueller, C;Johansen, JV;Helin, K;Lerdrup, M;Jensen, KB; An organoid-based CRISPR-Cas9 screen for regulators of intestinal epithelial maturation and cell fate Science advances 2023-07-14 [PMID: 37436979] (IHC, Mouse)
• Atreyi Biswas, Shailendra Kumar Singh, Gayathri M. Kartha, Satish Khurana Immuno-localization of definitive hematopoietic stem cells in the vascular niche of mouse fetal liver STAR Protocols 2022-12-16 [PMID: 36223268]
• Jessica Cristina Marín-Llera, Carlos Ignacio Lorda-Diez, Juan Mario Hurle, Jesús Chimal-Monroy SCA-1/Ly6A Mesodermal Skeletal Progenitor Subpopulations Reveal Differential Commitment of Early Limb Bud Cells Frontiers in Cell and Developmental Biology 2021-07-16 [PMID: 34336823]
• JDG Leach, N Vlahov, P Tsantoulis, RA Ridgway, DJ Flanagan, K Gilroy, N Sphyris, EG Vázquez, DF Vincent, WJ Faller, MC Hodder, A Raven, S Fey, AK Najumudeen, D Strathdee, C Nixon, M Hughes, W Clark, R Shaw, S:CORT con, SR van Hooff, DJ Huels, JP Medema, ST Barry, MC Frame, A Unciti-Bro, SJ Leedham, GJ Inman, R Jackstadt, BJ Thompson, AD Campbell, S Tejpar, OJ Sansom Oncogenic BRAF, unrestrained by TGF&beta-receptor signalling, drives right-sided colonic tumorigenesis Nature Communications, 2021-06-08;12(1):3464. 2021-06-08 [PMID: 34103493] (IHC, Mouse)
• T Sato, M Sase, S Ishikawa, M Kajita, J Asano, T Sato, Y Mori, T Ohteki Characterization of radioresistant epithelial stem cell heterogeneity in the damaged mouse intestine Sci Rep, 2020-05-22;10(1):8308. 2020-05-22 [PMID: 32444673] (IHC, Mouse)
• S Yui, L Azzolin, M Maimets, MT Pedersen, RP Fordham, SL Hansen, HL Larsen, J Guiu, MRP Alves, CF Rundsten, JV Johansen, Y Li, CD Madsen, T Nakamura, M Watanabe, OH Nielsen, PJ Schweiger, S Piccolo, KB Jensen YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration Cell Stem Cell, 2017-12-14;22(1):35-49.e7. 2017-12-14 [PMID: 29249464] (IHC, Mouse)
• Sergejeva S, Johansson AK, Malmhall C, Lotvall J Allergen exposure-induced differences in CD34+ cell phenotype: relationship to eosinophilopoietic responses in different compartments. Blood, 2003-09-25;103(4):1270-7. 2003-09-25 [PMID: 14512324] (ICC, Mouse)

Bioinformatics

• Gene Symbol: Ly6a
• Uniprot:
  • CAA28351()