SATB2 Antibody - BSA Free

Western Blot: SATB2 Antibody [NBP1-76913] - A20 cell lysate with SATB2 antibody at (A) 2 and (B) 4 ug/mL.

Immunocytochemistry/Immunofluorescence: SATB2 Antibody [NBP1-76913] - Immunofluorescence of SATB2 in Human Brain cells with SATB2 antibody at 50 ug/mL.

Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-76913] - Human brain with SATB2 antibody at 5 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

SATB2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit SATB2 Antibody - BSA Free (NBP1-76913) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of the human SATB2. The immunogen is located within amino acids 570 - 620 of SATB2. Amino Acid Squence: SQPAKESSPPREEA
• Specificity: At least two isoforms of SATB2 are known to exist. This SATB2 antibody will not cross-react with SATB1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SATB2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 2-4 ug/ml
• Control Peptide:

  SATB2 Peptide (NBP1-76913PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for SATB2 Antibody - BSA Free

• DNA-binding protein SATB2
• FLJ32076
• KIAA1034FLJ21474
• MGC119474
• MGC119477
• SATB family member 2
• SATB homeobox 2
• Special AT-rich sequence-binding protein 2

Background

Human special AT-rich sequence-binding protein-2 (SATB2) is a nuclear matrix/scaffold-associated region DNA-binding protein. Like its homolog SATB1, SATB2 selectively binds double-stranded, special AT-rich DNA sequences, but is expressed primarily in a subset of postmitotic, differentiating neurons in the neocortex. Mice deficient in SATB exhibit craniofacial abnormalities and defects in osteoblast differentiation and function. SATB2 also interacts with and enhances the activity of Runx2 and ATF4, two transcription factors that regulate osteoblast differentiation, indicating that SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. Recent experiments have shown that SATB2 interacts with histone deacetylase 1 and metastasis-associated protein 2, two proteins that are involved in chromatin remodeling, suggesting that SATB2 may also be involved in mediating epigenetic influences during cortical development. At least two isoforms of SATB2 are known to exist. This SATB2 antibody will not cross-react with SATB1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SATB2
• Uniprot:
  • Q9UPW6(Human)