SATB2 Antibody

Images

 
Western Blot: SATB2 Antibody [NBP1-76912] - Mouse brain tissue lysate with SATB2 antibody at (A) 2 and (B) 4 ug/mL.
Immunohistochemistry-Paraffin: SATB2 Antibody [NBP1-76912] - Human brain with SATB2 antibody at 5 ug/ml.
Chromatin Immunoprecipitation: SATB2 Antibody [NBP1-76912] - ChIP on Bcl2 promoter using rabbit IgG as control. Image from verified customer review.
Immunofluorescence: SATB2 Antibody [NBP1-76912] - Immunofluorescence of SATB2 in Human Brain cells with SATB2 antibody at 20 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ChIP, ELISA, IHC, IHC-P, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

SATB2 Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of the human SATB2. The immunogen is located within the first 50 amino acids of SATB2.
Specificity
At least two isoforms of SATB2 are known to exist. This SATB2 antibody will not cross-react with SATB1.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SATB2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 2-4 ug/ml
  • Chromatin Immunoprecipitation
  • ELISA 1:100-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Immunofluorescence 20 ug/ml
Positive Control
SATB2 Lysate (NBP2-65726)
Control Peptide
SATB2 Peptide (NBP1-76912PEP)
Reviewed Applications
Read 1 Review rated 4
using
NBP1-76912 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SATB2 Antibody

  • DNA-binding protein SATB2
  • FLJ32076
  • KIAA1034FLJ21474
  • MGC119474
  • MGC119477
  • SATB family member 2
  • SATB homeobox 2
  • Special AT-rich sequence-binding protein 2

Background

Human special AT-rich sequence-binding protein-2 (SATB2) is a nuclear matrix/scaffold-associated region DNA-binding protein. Like its homolog SATB1, SATB2 selectively binds double-stranded, special AT-rich DNA sequences, but is expressed primarily in a subset of postmitotic, differentiating neurons in the neocortex. Mice deficient in SATB exhibit craniofacial abnormalities and defects in osteoblast differentiation and function. SATB2 also interacts with and enhances the activity of Runx2 and ATF4, two transcription factors that regulate osteoblast differentiation, indicating that SATB2 acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation. Recent experiments have shown that SATB2 interacts with histone deacetylase 1 and metastasis-associated protein 2, two proteins that are involved in chromatin remodeling, suggesting that SATB2 may also be involved in mediating epigenetic influences during cortical development. At least two isoforms of SATB2 are known to exist. This SATB2 antibody will not cross-react with SATB1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SATB2 Antibody (NBP1-76912) (0)

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Review for SATB2 Antibody (NBP1-76912) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-76912:
Filter by Applications
ChIP
(1)
All Applications
Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
Chromatin Immunoprecipitation SATB2 NBP1-76912
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reviewed by:
Ranveer Singh
ChIP Human 06/25/2014
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Summary

ApplicationChromatin Immunoprecipitation
Sample TestedCross-linked chromatin from HEK 293T cells
SpeciesHuman

Primary Anitbody

Dilution Ratio2ug for 400 ul lysate

Details

Detection Notesreal time quantitative PCR (SYBR green)
Fixation/Cross-LinkingReverse cross linking was done in elution buffer with NaCl at 65 degree O/N
DNS SharingDiagenode Bioruptor was used in "High" setting, for 7 mins X 3 time to get approx 500bp fragments
Immunoprecipitation DetailsMagnetic Dynabeads (Protein G)
Crosslinking/Reversal Details1% formaldehyde for 10 mins at room temperature
DNA Prurification DetailsDNA was purified using PCR Purification Kit (Qiagen)

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ChIP Video Protocol
ChIP Webinar

FAQs for SATB2 Antibody (NBP1-76912) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SATB2 Antibody (NBP1-76912)

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Diseases for SATB2 Antibody (NBP1-76912)

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PTMs for SATB2 Antibody (NBP1-76912)

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Blogs on SATB2

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Ranveer Singh
06/25/2014
Application: ChIP
Species: Human

Bioinformatics

Gene Symbol SATB2
Uniprot