SARM1 Antibody

Immunocytochemistry/Immunofluorescence: SARM1 Antibody [NBP1-87897] - Staining of human cell line SiHa shows localization to vesicles. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: SARM1 Antibody [NBP1-87897] - Staining of human testis shows strong cytoplasmic positivity in Leydig cells.

Immunohistochemistry-Paraffin: SARM1 Antibody [NBP1-87897] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: SARM1 Antibody [NBP1-87897] - Staining of human liver shows strong cytoplasmic positivity in bile duct cells.

Immunohistochemistry-Paraffin: SARM1 Antibody [NBP1-87897] - Staining of human prostate shows no cytoplasmic positivity in glandular cells as expected.

Product Discontinued

SARM1 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: LATNKEVEREVERSGTLALVEPLVASLDPGRFARCLVDASDTSQGRGPDDLQRLVPLLDSNRLEAQCIGAFYLCAEAAIKSLQGKTKVFS
• Predicted Species: Mouse (96%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SARM1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for SARM1 Antibody

• KIAA0524SAM domain-containing protein 2
• SAMD2
• SAMD2sterile alpha and HEAT/Armadillo motif protein, ortholog of Drosophila
• SARM1
• SARMFLJ36296
• Sterile alpha and Armadillo repeat protein
• sterile alpha and TIR motif containing 1
• sterile alpha and TIR motif-containing protein 1
• Sterile alpha motif domain-containing protein 2
• Tir-1 homolog

Background

Toll-like receptors (TLRs) play an essential role in the detection and elimination of invading microbes. They are type-1 transmembrane receptors, containing extracellular leucine rich repeats and an intracellular Toll/interleukin-1 receptor (TIR) domain. Upon stimulation, these receptors interact with specific TIR domain-containing adaptor proteins. Five such adaptors have been discovered to date, MyD88, Mal (MyD88 adaptor-like)/TIRAP (TIR domain-containing adaptor protein), Trif (TIR-domain-containing adaptor inducing interferon-beta), TRAM (Trif-related adaptor molecule) and SARM (SAM and ARM-containing protein). Different TLRs use different combinations of these adaptors, leading to the activation of common and unique pathways involved in the elimination of the invading microbe.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SARM1