SAE2 Antibody (OTI4A3) [mFluor Violet 450 SE]

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm
• Applications: WB, ICC/IF, IHC
• Clone: OTI4A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 450 SE

SAE2 Antibody (OTI4A3) [mFluor Violet 450 SE] Summary

• Immunogen: Full length human recombinant protein of human UBA2 (NP_005490) produced in HEK293T cell.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: UBA2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for SAE2 Antibody (OTI4A3) [mFluor Violet 450 SE]

• Anthracycline-associated resistance ARX
• ARX
• EC 6.3.2
• EC 6.3.2.-
• FLJ13058
• HRIHFB2115
• SAE2SUMO-1 activating enzyme subunit 2
• SUMO1 activating enzyme subunit 2
• SUMO-activating enzyme subunit 2
• UBA2, ubiquitin-activating enzyme E1 homolog
• Ubiquitin-like 1-activating enzyme E1B
• ubiquitin-like modifier activating enzyme 2
• UBLE1B

Background

Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes. The conjugation of SUMO-1, SUMO-2 and SUMO-3 onto target proteins requires the concerted action of the specific E1-activating enzyme SAE1/SAE2, the E2-conjugating enzyme Ubc9, and an E3-like SUMO ligase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBA2