SAE2 Antibody (JM66-53)

Western Blot: SAE2 Antibody (JM66-53) [NBP2-75654] - Analysis of SAE2/UBA2 on A549 (1) and PC-12 (2) cell lysate using anti-SAE2/UBA2 antibody at 1/500 dilution.

Product Discontinued

SAE2 Antibody (JM66-53) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human SAE2 aa 39-88 / 640. (SwissProt: Q9UBT2 Human; SwissProt: Q9Z1F9 Mouse; SwissProt: B1WC32 Rat)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: UBA2
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1:10-1:50
  • Western Blot 1:500
• Theoretical MW: 71 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for SAE2 Antibody (JM66-53)

• Anthracycline-associated resistance ARX
• ARX
• EC 6.3.2
• EC 6.3.2.-
• FLJ13058
• HRIHFB2115
• SAE2SUMO-1 activating enzyme subunit 2
• SUMO1 activating enzyme subunit 2
• SUMO-activating enzyme subunit 2
• UBA2, ubiquitin-activating enzyme E1 homolog
• Ubiquitin-like 1-activating enzyme E1B
• ubiquitin-like modifier activating enzyme 2
• UBLE1B

Background

Covalent attachment of one protein to another is one of the more prominent posttranslational modifications in respect to size and ubiquity. Ubiquitin is the most familiar of the protein modifiers and its activation and transfer to target proteins has been studied extensively. Recently, a new group of ubiquitin-like proteins has been receiving a lot of attention. SUMO, or Sentrin, is one of the most intriguing. SUMO has been implicated in the stabilization of target proteins and/or their localization to sub-cellular complexes. The conjugation of SUMO-1, SUMO-2 and SUMO-3 onto target proteins requires the concerted action of the specific E1-activating enzyme SAE1/SAE2, the E2-conjugating enzyme Ubc9, and an E3-like SUMO ligase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: UBA2