SA1 Antibody

Images

 
Western Blot: SA1 Antibody [NB100-299] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-SA1 ...read more
Immunocytochemistry: SA1 Antibody [NB100-299] - HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.
Western Blot: SA1 Antibody [NB100-299] - Nuclear extract from approx. 500,000 HeLa cells. Antibody used at 0.5 ug/ml.

Product Details

Summary
Reactivity Hu, XpSpecies Glossary
Applications WB, ICC/IF, ICC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

SA1 Antibody Summary

Immunogen
A 35 amino acid synthetic peptide, which represented a portion of human Stromal Antigen 1 encoded in part by exons 35 and 36 (LocusLink ID 10274).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
STAG1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunocytochemistry/Immunofluorescence 1:500-1:2000
  • Immunocytochemistry
Application Notes
NB 100-299 may be used for Western Blot where a band at ~145 kD is seen on SDS-PAGE. It may also be used for immunocytochemistry. *The investigator should determine the optimal working dilution for a specific application.
Positive Control
HeLa Lysate (NB800-PC9)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SA1 Antibody

  • cohesin subunit SA-1
  • nuclear protein stromal antigen 1
  • SA1
  • stromal antigen 1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SA1 Antibody (NB100-299) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional SA1 Products

Bioinformatics Tool for SA1 Antibody (NB100-299)

Discover related pathways, diseases and genes to SA1 Antibody (NB100-299). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for SA1 Antibody (NB100-299)

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Bioinformatics

Gene Symbol STAG1