SA1 Antibody - BSA Free

Western Blot: SA1 Antibody [NB100-299] - Detection of human SA1 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-SA1 antibody NB100-299 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunocytochemistry/Immunofluorescence: SA1 Antibody [NB100-299] - HeLa cells that were extracted for 5 min. at 4C in 0.5% Triton in CSK buffer. Antibody used at 1 ug/ml.

Western Blot: SA1 Antibody [NB100-299] - Nuclear extract from approx. 500,000 HeLa cells. Antibody used at 0.5 ug/ml.

Western Blot: SA1 Antibody [NB100-299] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-SA1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation: SA1 Antibody [NB100-299] - Detection of human SA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-SA1 antibody NB100-299 (lot NB100-299-3) used for IP at 6 ug per reaction. SA1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-299-2). For blotting immunoprecipitated SA1, NB100-299 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

SA1 Antibody - BSA Free Summary

• Description: Novus Biologicals Goat SA1 Antibody - BSA Free (NB100-299) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A 35 amino acid synthetic peptide, which represented a portion of human Stromal Antigen 1 encoded in part by exons 35 and 36 (LocusLink ID 10274).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: STAG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500-1:2000
  • Immunoprecipitation
  • Western Blot 1:1000-1:10000
• Application Notes: NB 100-299 may be used for Western Blot where a band at ~145 kD is seen on SDS-PAGE. It may also be used for immunocytochemistry. *The investigator should determine the optimal working dilution for a specific application.
• Control:

  HeLa Nuclear Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for SA1 Antibody - BSA Free

• cohesin subunit SA-1
• nuclear protein stromal antigen 1
• SA1
• stromal antigen 1

Background

SA1 is a member of the SCC3 family and is expressed in the nucleus. It encodes a component of cohesin, a multisubunit protein complex that provides sister chromatid cohesion along the length of a chromosome from DNA replication through prophase and prometaphase, after which it is dissociated in preparation for segregation during anaphase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: STAG1