S5a/Angiocidin Antibody

Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse S5a/Angiocidin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5540) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S5a/Angiocidin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

S5a/Angiocidin Antibody Summary

• Immunogen: E. coli-derived recombinant mouse S5a/Angiocidin isoform A
  Val2-Glu254
  Accession # O35226
• Specificity: Detects mouse S5a/Angiocidin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PSMD4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S5a/Angiocidin Antibody

• 26S proteasome non-ATPase regulatory subunit 4;AF;AF-1;ASF;MCB1;PSMD4;pUB-R5;Rpn10;S5A
• Angiocidin
• ASF
• Macropain
• Mcb1
• PSMD4
• pUB-R5
• Rpn10
• S5a

Background

Angiocidin (also S5a, 26S proteasome regulatory subunit S5A, Psmd4, RPN10a and Mcb1) is a 41 kDa (predicted) member of the proteosome subunit S5A family of proteins. It runs anomalously in SDS-PAGE at 55 kDa. Intracellularly, Angiocidin is a component of the 26S proteosome that degrades ubiquitinated proteins. Extracellularly, it inhibits angiogenesis by binding to collagen and beta 1 integrins. Mouse Angiocidin is 376 amino acids (aa) in length. It contains a vWF-A domain (aa 5‑188), a beta 1 interacting site (aa 86‑105) and two ubiquitin-interacting motifs (aa 211‑230 and 282‑301). There are four splice variants. Three show a GlyGluArg insertion after Glu254, with two of these showing an additional three aa substitution for aa 255‑376, and a 45 aa substitution for aa 321‑376, respectfully. A fourth shows a 51 aa substitution for aa 299‑376. Over aa 1‑254, mouse Angiocidin shares > 99% aa identity with rat and human Angiocidin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S5a/Angiocidin Antibody (AF5540)(1)

Publication using AF5540

• JJ Yi, SR Paranjape, MP Walker, R Choudhury, JM Wolter, G Fragola, MJ Emanuele, MB Major, MJ Zylka The autism-linked UBE3A T485A mutant E3 ubiquitin ligase activates the Wnt/?-catenin pathway by inhibiting the proteasome J. Biol. Chem., 2017-05-30;0(0):. 2017-05-30 [PMID: 28559284] (Western Blot, Human)

Bioinformatics

• Gene Symbol: PSMD4
• Uniprot:
  • O35226()