S100A8/S100A9 Heterodimer Antibody (900028) [Alexa Fluor™ Plus 555]

• Reactivity: Hu
• Clone: 900028
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 555

S100A8/S100A9 Heterodimer Antibody (900028) [Alexa Fluor™ Plus 555] Summary

• Specificity: Detects human S100A8/S100A9 heterodimer in direct ELISAs. In direct ELISAs, no detection of recombinant human S100A8 or S100A9 monomers was observered.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100A8/S100A9 Heterodimer Antibody (900028) [Alexa Fluor™ Plus 555]

• 60B8AG
• CAGA
• calgranulin A
• Calprotectin L1L subunit
• Calprotectin
• CFAG
• CGLA
• CP-10
• Cystic fibrosis antigen
• L1AG
• Leukocyte L1 complex light chain
• MA387
• MIF
• Migration inhibitory factor-related protein 8
• MRP8
• NIF
• p8
• protein S100-A8
• S100-A8
• S100A8/S100A9 Heterodimer
• Urinary stone protein band A

Background

S100A8 (also known as MRP8, Calgranulin A, and CP-10) and S100A9 (also known as MRP14 and Calgranulin B) are pro-inflammatory members of the S100 family of secreted calcium binding proteins (1, 2). They are up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and are present at elevated concentrations in rheumatoid arthritis synovial fluid (3-5). The 10 kDa human S100A8 and 14 kDa S100A9 each contain two EF‑hand calcium binding motifs. Human S100A8 shares 57% and 61% amino acid (aa) sequence identity with mouse and rat S100A8, respectively. Human S100A9 shares 57% and 62% amino acid sequence identity with mouse and rat S100A9, respectively (6, 7). In the presence of calcium or zinc, S100A8 and S100A9 associate into non-covalent homodimers and 34-35 kDa heterodimers with each other (8-10). The heterodimer additionally binds and sequesters manganese, thereby restricting the growth of Mn-dependent bacteria (11). The S100A8/A9 heterodimer exhibits functions beyond those performed by the individual proteins. These include binding to fatty acids such as arachidonic acid and promoting astrocyte proliferation (3, 12). S100A8, S100A9, and the heterodimer each promote neutrophil infiltration into sites of inflammation and inflammatory cytokine production by monocytes (4, 5, 9).

1. Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223.
2. Vogl, T. et al. (2012) Int. J. Mol. Sci. 13:2893.
3. Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371.
4. Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69.
5. Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250.
6. Odink, K. et al. (1987) Nature 330:80.
7. Dorin, J.R. et al. (1987) Nature 326:614.
8. Teigelkamp, S. et al. (1991) J. Biol. Chem. 266:13462.
9. Ryckman, C. et al. (2003) J. Immunol. 170:3233.
10. Vogl, T. et al. (2006) Biochim. Biophys. Acta 1763:1298.
11. Damo, S.M. et al. (2013) Proc. Natl. Acad. Sci. USA 110:3841.
12. Ryu, M-J. et al. (2012) J. Biol. Chem. 287:22948.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.