S100A2 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human S100A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4870) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S100A2 at approximately 8 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

S100A2 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-Human S100A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4870) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI(blue). Specific staining was localized to nucleus and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

S100A2 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human S100A2
  Met2-Pro98
  Accession # P29034
• Specificity: Detects human S100A2 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) S100A1, -A6, -A7, -A8, -A10, -A11, -A13, -A16, rhS100B and rhS100P is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: S100A2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100A2 Antibody [Unconjugated]

• CAN19
• CAN19protein S100-A2
• MGC111539
• S100 calcium binding protein A2
• S100 calcium-binding protein A2Protein S-100L
• S100A2
• S100L

Background

S100A2 (also S100L and CaN19) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is expressed by multiple cell types including keratinocytes, chondrocytes, and bronchial epithelium. S100A2 regulates both Ca and Zn (in human) within cells and increases p53 activity. It forms both noncovalent and covalent homodimers and a homotetramer under certain conditions. Human S100A2 is 98 amino acids (aa) in length. It contains two EF-hand motifs (aa 13‑48 and 51‑86) plus a calcium (aa 64‑75) and zinc (aa 17‑22) binding site. There is one potential alternative splice form that shows a 40 aa insertion after Gly48. Full-length human S100A2 shares 89% and 85% aa identity with bovine and canine S100A2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S100A2 Antibody (AF4870)(1)

Publication using AF4870

• Shimamoto , Seiko, Kubota , Yasuo, Yamaguchi , Fuminori, Tokumitsu , Hiroshi, Kobayashi , Ryoji Ca2+/S100 proteins act as upstream regulators of the chaperone-associated ubiquitin ligase CHIP (C terminus of Hsc70-interacting protein). J Biol Chem, 2013-01-23;288(10):7158-68. 2013-01-23 [PMID: 23344957] (Western Blot, Human)

Bioinformatics

• Gene Symbol: S100A2
• Uniprot:
  • P29034()