RXR beta/NR2B2 Antibody

Western Blot: RXR beta/NR2B2 Antibody [NB100-1467] - Staining (1ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western Blot: RXR beta/NR2B2 Antibody [NB100-1467] - (1ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western Blot: RXR beta/NR2B2 Antibody [NB100-1467] - HEK293 overexpressing RXRB and probed with (mock transfection in first lane).

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

RXR beta/NR2B2 Antibody Summary

• Immunogen: Peptide with sequence CRDGMGDSGRDSRSP corresponding to internal region according to NP_068811.1.
• Specificity: This antibody is expected to recognise an epitope corresponding to aa 70-83 of human RXR beta protein and does not cross-react with either RXR alpha or gamma.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: RXRB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 55 kDa band observed in A431 lysates (calculated MW of 56.9 kDa band according to NP_068811.1). In transfected HEK293 transiently expressing RXRB a band of approx. 60 kDa is observed. This band is not observed in the non-transfected HEK293.
• Control:

  RXR beta/NR2B2 Overexpression Lysate

• Control Peptide:

  RXR beta/NR2B2 Peptide (NB100-1467PEP)

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RXR beta/NR2B2 Antibody

• DAUDI6
• H-2RIIBP
• MHC class I promoter binding protein
• NR2B2
• NR2B2MGC1831
• Nuclear receptor subfamily 2 group B member 2
• RCoR-1
• retinoic acid receptor RXR-beta
• Retinoid X receptor beta
• retinoid X receptor, beta
• RXR beta
• RXRB

Background

Retinoid X receptors (RXRs) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. The RXR subfamily consists of at least three similar genes, RXR alpha, RXR beta and RXR gamma, all of which control transcription of target genes mediated by retinoids. RXR beta controls expression of many genes that respond to hormones and vitamins, including thyroid hormone, estrogen, retinoids and vitamin D. RXR beta controls a wide array of genes because of its ability to heterodimerize with other hormone receptors including the thyroid hormone receptor (THR), vitamin D receptor (VDR) and retinoic acid receptor (RAR).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for RXR beta/NR2B2 Antibody (NB100-1467)(1)

Publication using NB100-1467

• Samad TA, Krezel W, Chambon P, Borrelli E. Regulation of dopaminergic pathways by retinoids: activation of the D2 receptor promoter by members of the retinoic acid receptor-retinoid X receptor family. Proc Natl Acad Sci U S A 1997-12-23 [PMID: 9405615]

Bioinformatics

• Gene Symbol: RXRB
• Entrez:
  • 6257(Human)
• Uniprot:
  • NP_068811.(Human)