![Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Western blot analysis of RXR alpha/NR2B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: MCF-7 cell lysate](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Western-Blot-NBP2-75653-img0006.jpg "Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Western blot analysis of RXR alpha/NR2B1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: MCF-7 cell lysate")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | JG99-38 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody |
| Immunogen | Recombinant protein corresponding to Human RXR alpha/NR2B1 aa 1-50 / 462. (SwissProt: P19793 Human; SwissProt: P28700 Mouse; SwissProt: Q05343 Rat) |
| Localization | Mitochondrion, Nucleus, Cytoplasm. |
| Source | HEK293 |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | RXRA |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 51 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
![RXR gamma/NR2B3 Antibody (H3210) [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "RXR gamma/NR2B3 Antibody (H3210) [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Research Areas for RXR alpha/NR2B1 Antibody (NBP2-75653)Find related products by research area.
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| Gene Symbol | RXRA |
![Immunocytochemistry/Immunofluorescence: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Staining Retinoid X Receptor alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Immunocytochemistry-Immunofluorescence-NBP2-75653-img0002.jpg "Immunocytochemistry/Immunofluorescence: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Staining Retinoid X Receptor alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Analysis of paraffin-embedded rat liver tissue using anti-Retinoid X Receptor alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Immunohistochemistry-Paraffin-NBP2-75653-img0004.jpg "Immunohistochemistry-Paraffin: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Analysis of paraffin-embedded rat liver tissue using anti-Retinoid X Receptor alpha antibody. Counter stained with hematoxylin.")
![Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Analysis of Retinoid X Receptor alpha on MCF-7 cell using anti-Retinoid X Receptor alpha antibody at 1/5,000 dilution.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Western-Blot-NBP2-75653-img0005.jpg "Western Blot: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Analysis of Retinoid X Receptor alpha on MCF-7 cell using anti-Retinoid X Receptor alpha antibody at 1/5,000 dilution.")
![Immunocytochemistry/Immunofluorescence: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Staining Retinoid X Receptor alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Immunocytochemistry-Immunofluorescence-NBP2-75653-img0001.jpg "Immunocytochemistry/Immunofluorescence: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Staining Retinoid X Receptor alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Analysis of paraffin-embedded human esophagus tissue using anti-Retinoid X Receptor alpha antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/RXR-alpha-NR2B1-Antibody-JG99-38-Immunohistochemistry-Paraffin-NBP2-75653-img0003.jpg "Immunohistochemistry-Paraffin: RXR alpha/NR2B1 Antibody (JG99-38) [NBP2-75653] - Analysis of paraffin-embedded human esophagus tissue using anti-Retinoid X Receptor alpha antibody. Counter stained with hematoxylin.")
![Bioactivity IL-2 [Unconjugated]](https://images.novusbio.com/images/202-il_recombinant-human-il-2-protein-bioactivity-174202314946.jpg)
![N/A IL-10 [Biotin]](https://images.novusbio.com/images2/DATA_IL10_DY417_ELISA_2014.jpg)
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")