RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6)

Immunocytochemistry/Immunofluorescence: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) [NBP3-07586] - Immunofluorescence Analysis of PFA-fixed K562 cells using RXR beta/NR2B2 Mouse Monoclonal Antibody (PCRP-RXRB-2B6) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).

Immunohistochemistry-Paraffin: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) [NBP3-07586] - Formalin-fixed, paraffin-embedded human lymph node stained with RXR beta/NR2B2 Mouse Monoclonal Antibody (PCRP-RXR beta/NR2B2-2B6).

Flow Cytometry: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) [NBP3-07586] - Flow Cytometric Analysis of PFA-fixed U87 cells. RXR beta/NR2B2 Mouse Monoclonal Antibody (PCRP-RXRB-2B6) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).

Immunocytochemistry/Immunofluorescence: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) [NBP3-07586] - Immunofluorescence Analysis of PFA-fixed HeLa cells using RXR beta/NR2B2 Mouse Monoclonal Antibody (PCRP-RXRB-2B6) followed by goat anti- Mouse IgG-CF488 (green). CF640A phalloidin (red).

Protein Array: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) [NBP3-07586] - Analysis of Protein Array containing more than 19,000 full-length human proteins using RXR beta/NR2B2 Mouse Monoclonal Antibody (PCRP-RXR beta/NR2B2-2B6).

Flow Cytometry: RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) [NBP3-07586] - Flow Cytometric Analysis of PFA-fixed HeLa cells. RXR beta/NR2B2 Mouse Monoclonal Antibody (PCRP-RXR beta/NR2B2-2B6) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, MA
• Clone: PCRP-RXRB-2B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08445)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human RXR beta/NR2B2 protein (Uniprot: P28702)
• Localization: Nucleus.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: RXRB
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for RXR beta/NR2B2 Antibody (PCRP-RXRB-2B6)

• DAUDI6
• H-2RIIBP
• MHC class I promoter binding protein
• NR2B2
• NR2B2MGC1831
• Nuclear receptor subfamily 2 group B member 2
• RCoR-1
• retinoic acid receptor RXR-beta
• Retinoid X receptor beta
• retinoid X receptor, beta
• RXR beta
• RXRB

Background

Retinoid X receptors (RXRs) are members of the steroid/thyroid hormone receptor superfamily of nuclear receptor proteins which exert their effects by binding to specific DNA response elements, thus regulating gene expression in target cells. The RXR subfamily consists of at least three similar genes, RXR alpha, RXR beta and RXR gamma, all of which control transcription of target genes mediated by retinoids. RXR beta controls expression of many genes that respond to hormones and vitamins, including thyroid hormone, estrogen, retinoids and vitamin D. RXR beta controls a wide array of genes because of its ability to heterodimerize with other hormone receptors including the thyroid hormone receptor (THR), vitamin D receptor (VDR) and retinoic acid receptor (RAR).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RXRB
• Uniprot:
  • P28702(Human)