RUNX3/CBFA3 Antibody (BLR122H)

Immunoprecipitation: RUNX3/CBFA3 Antibody [NBP3-14706] - Whole cell lysate (1.0 mgper IP reaction; 20% of IP loaded) from SK-MEL-28 cellsprepared using NETN lysis buffer. Antibodies: Rabbit antiRUNX3 recombinant monoclonal antibody used for IP at 20 ul/mg lysate. RUNX3was also immunoprecipitated by rabbit anti-RUNX3antibody. For blotting immunoprecipitatedRUNX3, was used at 1:1000.Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: BLR122H
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

RUNX3/CBFA3 Antibody (BLR122H) Summary

• Additional Information: Recombinant Monoclonal
• Immunogen: between 1 and 50
• Clonality: Monoclonal
• Host: Rabbit
• Gene: RUNX3
• Purity: >95%

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 2 - 8 C / 1 year from date of receipt
• Buffer: Borate Buffered Saline (BBS) pH 8.2 with 0.1% BSA and
• Preservative: 0.09% Sodium Azide
• Purity: >95%

Alternate Names for RUNX3/CBFA3 Antibody (BLR122H)

• Acute myeloid leukemia 2 protein
• AML2
• AML2SL3/AKV core-binding factor alpha C subunit
• CBFA3
• CBFA3MGC16070
• CBF-alpha-3
• PEA2 alpha C
• PEA2-alpha C
• PEBP2 alpha C
• PEBP2A3
• PEBP2A3FLJ34510
• PEBP2AC
• PEBP2-alpha C
• runt domain, alpha subunit 3
• runt-related transcription factor 3
• RUNX3
• SL3-3 enhancer factor 1 alpha C subunit
• transcription factor AML2

Background

RUNX3 binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. RUNX3 consists of a heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer through the Runt domain. DNA-binding is increased by heterodimerization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RUNX3