CACNA1G Antibody (S178A-9) - BSA Free

Western Blot: CACNA1G Antibody (S178A-9) [NBP2-59322] - Western Blot analysis of Rat brain membrane lysate showing detection of CACNA1G Calcium Channel protein using Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Primary Antibody: Mouse Anti-CACNA1G Calcium Channel Monoclonal Antibody (NBP2-59322) at 1:1000.

Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CACNA1G Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-CACNA1G Monoclonal Antibody (NBP2-59322) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) CACNA1G Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Tissue: SK-N-BE Cells (Human Neuroblastoma cells). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav3.1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X.

Immunocytochemistry/Immunofluorescence: CACNA1G Antibody (S178A-9) [NBP2-59322] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CACNA1G Monoclonal Antibody, Clone S178A-9 (NBP2-59322). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CACNA1G Monoclonal Antibody (NBP2-59322) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) CACNA1G Antibody. (D) Composite.

Western Blot: Mouse Monoclonal CACNA1G Antibody (S178A-9) [NBP2-59322] - Western blot using lysates from human primary astrocytes or IGR39 melanoma cell line. Image from a verified customer review.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: S178A-9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

CACNA1G Antibody (S178A-9) - BSA Free Summary

• Description: Novus Biologicals Mouse CACNA1G Antibody (S178A-9) - BSA Free (NBP2-59322) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Fusion protein amino acids 2052-2172 (cytoplasmic C-terminus) of mouse Cav3.1
• Localization: Membrane
• Specificity: Detects <200kDa. Does not cross-react with Cav3.2.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CACNA1G
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry
  • Western Blot 1:1000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein G purified

Alternate Names for CACNA1G Antibody (S178A-9) - BSA Free

• CACNA1G
• calcium channel, voltage-dependent, T type, alpha 1G subunit
• Cav3.1c
• KIAA1123
• NBR13
• voltage-dependent calcium channel alpha 1G subunit
• voltage-dependent T-type calcium channel subunit alpha-1G
• voltage-dependent, alpha 1G subunit
• voltage-dependent, T type, alpha-1G subunit

Background

Voltage-activated calcium channels can be distinguished based on their voltage-dependence, deactivation, and single-channel conductance. See MIM 601011. Low-voltage-activated calcium channels are referred to as 'T' type because their currents are both transient, owing to fast inactivation, and tiny, owing to small conductance. T-type channels are thought to be involved in pacemaker activity, low-threshold calcium spikes, neuronal oscillations and resonance, and rebound burst firing.(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CACNA1G Antibody (NBP2-59322)(1)

Publication using NBP2-59322

• Odamah K, Man H Restoration of NEXMIF expression rescues abnormalities in gene transcription, neuron maturation and autistic-like behaviors in Nexmif knockout mice. Translational Psychiatry 2025-10-06 [PMID: 41053001]

Review for CACNA1G Antibody (NBP2-59322) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Pauline Jeannot WB Human 11/16/2023

Summary

• Application: Western Blot
• Sample Tested: IGR39 melanoma cell line
• Species: Human
• Lot: 1205

Bioinformatics

• Gene Symbol: CACNA1G