RUNX3/CBFA3 Antibody (2B3) - BSA Free

Western Blot: RUNX3/CBFA3 Antibody (2B3) [NBP2-37455] - Western blot analysis using RUNX3 mAb against HEK293 (1) and RUNX3 (AA: 186-252)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemistry: RUNX3/CBFA3 Antibody (2B3) [NBP2-37455] - Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using RUNX3 mouse mAb with DAB staining.

Flow Cytometry: RUNX3/CBFA3 Antibody (2B3) [NBP2-37455] - Flow cytometric analysis of NIH3T3 cells using RUNX3 mouse mAb (green) and negative control (red).

Western Blot: RUNX3/CBFA3 Antibody (2B3) [NBP2-37455] - Western blot analysis using RUNX3 mAb against human RUNX3 recombinant protein. (Expected MW is 33 kDa)

ELISA: RUNX3/CBFA3 Antibody (2B3) [NBP2-37455] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 2B3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

RUNX3/CBFA3 Antibody (2B3) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human RUNX3/CBFA3 (AA:186-252) expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: RUNX3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 44.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for RUNX3/CBFA3 Antibody (2B3) - BSA Free

• Acute myeloid leukemia 2 protein
• AML2
• AML2SL3/AKV core-binding factor alpha C subunit
• CBFA3
• CBFA3MGC16070
• CBF-alpha-3
• PEA2 alpha C
• PEA2-alpha C
• PEBP2 alpha C
• PEBP2A3
• PEBP2A3FLJ34510
• PEBP2AC
• PEBP2-alpha C
• runt domain, alpha subunit 3
• runt-related transcription factor 3
• RUNX3
• SL3-3 enhancer factor 1 alpha C subunit
• transcription factor AML2

Background

RUNX3 binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. RUNX3 consists of a heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer through the Runt domain. DNA-binding is increased by heterodimerization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RUNX3