RPTOR Antibody

Images

 
Western Blot: RPTOR Antibody [NBP2-89142] - Analysis of Raptor on MCF-7 cell lysate using anti-Raptor antibody at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence: RPTOR Antibody [NBP2-89142] - Staining Raptor in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% ...read more
Immunohistochemistry-Paraffin: RPTOR Antibody [NBP2-89142] - Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-RPTOR antibody. Counter stained with hematoxylin.
Flow Cytometry: RPTOR Antibody [NBP2-89142] - Analysis of A549 cells with Raptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor ...read more
Immunocytochemistry/ Immunofluorescence: RPTOR Antibody [NBP2-89142] - Staining Raptor in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% ...read more
Immunohistochemistry-Paraffin: RPTOR Antibody [NBP2-89142] - Analysis of paraffin-embedded rat brain tissue using anti-Raptor antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: RPTOR Antibody [NBP2-89142] - Analysis of paraffin-embedded human breast tissue using anti-Raptor antibody. Counter stained with hematoxylin.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

RPTOR Antibody Summary

Immunogen
Synthetic peptide within human RPTOR aa 90-170. (SwissProt: Q8N122 Human;SwissProt: Q8K4Q0 Mouse)
Localization
Cytoplasm. Lysosome.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RPTOR
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
Theoretical MW
131 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
1 x TBS (pH7.4), 0.5% BSA, 50% Glycerol.
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for RPTOR Antibody

  • KIAA1303
  • KIAA1303p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
  • KOG1
  • Mip1
  • p150 target of rapamycin (TOR)-scaffold protein
  • Raptor
  • regulatory associated protein of MTOR, complex 1
  • regulatory-associated protein of mTOR
  • RPTOR

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol RPTOR