RPTOR Antibody

Western Blot: RPTOR Antibody [NBP2-89142] - Analysis of Raptor on MCF-7 cell lysate using anti-Raptor antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: RPTOR Antibody [NBP2-89142] - Staining Raptor in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RPTOR Antibody [NBP2-89142] - Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-RPTOR antibody. Counter stained with hematoxylin.

Flow Cytometry: RPTOR Antibody [NBP2-89142] - Analysis of A549 cells with Raptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: RPTOR Antibody [NBP2-89142] - Staining Raptor in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: RPTOR Antibody [NBP2-89142] - Analysis of paraffin-embedded rat brain tissue using anti-Raptor antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: RPTOR Antibody [NBP2-89142] - Analysis of paraffin-embedded human breast tissue using anti-Raptor antibody. Counter stained with hematoxylin.

Product Discontinued

RPTOR Antibody Summary

• Immunogen: Synthetic peptide within human RPTOR aa 90-170. (SwissProt: Q8N122 Human; SwissProt: Q8K4Q0 Mouse)
• Localization: Cytoplasm. Lysosome.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: RPTOR
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 131 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for RPTOR Antibody

• KIAA1303
• KIAA1303p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats
• KOG1
• Mip1
• p150 target of rapamycin (TOR)-scaffold protein
• Raptor
• regulatory associated protein of MTOR, complex 1
• regulatory-associated protein of mTOR
• RPTOR

Background

Raptor (regulatory associated protein of TOR) is a conserved 150 kDa adaptor protein composed of a unique conserved region, three HEAT repeats and seven WD-40 repeats (1). Raptor recruits mTOR substrates S6K1 (p70 S6 kinase) and 4E-BP1, and regulates mTOR kinase activity (2-3). Even though Raptor is a positive regulator of mTOR, it has been shown that under nutrient deprivation, the Raptor-mTOR association is stabilized in a manner that inhibits mTOR kinase activity (1). Raptor association with mTOR is required for efficient S6K1 and 4E0BP1 phosphorylation (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: RPTOR